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Our observations and those of Halgren [ 4 ] suggest that a given force field or quantum chemical method selected for potential application to a highly-polar molecular system be carefully validated to assure that conformational energy comparisons are not spurious.
For example, IMAGE mouse cDNA clones (approximately 1200) were purchased from Research Genetics (Huntsville, Alabama) and sequences were verified by Halgren et al [ 11 ] . This group found that only 62% were definitely identified as a pure sample of the correct clones.