Words similar to gtp
Example sentences for: gtp
How can you use “gtp” in a sentence? Here are some example sentences to help you improve your vocabulary:
Rab proteins comprise a large family of Ras-related GTPases that function in the anterograde and retrograde trafficking of proteins in mammalian cells [ 1 2 3 ] . Different Rab proteins reside in specific subcellular membranes or organelles, where they mediate vesicular transport between discrete donor and acceptor compartments in the endocytic or exocytic pathways [ 4 5 ] . Detailed studies of a few Rab proteins ( e.g .; Rab1, Rab5 and Rab9) have led to a general model wherein Rab proteins are presumed to cycle on and off donor and acceptor membranes in connection with changes in their guanine nucleotide state [ 6 7 8 ] . In the active GTP-bound state, Rab proteins associate with budding transport vesicles [ 9 10 11 ] and appear to participate in the assembly of membrane docking complexes [ 12 13 14 ] . Following vesicle fusion with the acceptor compartment and GTP hydrolysis, the inactive GDP-bound Rab is extracted from the membrane by a carrier protein termed guanine nucleotide dissociation inhibitor (GDI) [ 15 16 17 ] . The GTPase can then re-enter the transport cycle through nucleotide exchange promoted by a specific exchange factor at the donor vesicle membrane [ 18 19 20 ] . In addition to changes in guanine nucleotide state, the posttranslational prenylation of Rab proteins plays an important role in the cycling mechanism.
In 2 mM GMPCPP, a slowly hydrolyzable analog of GTP, D209A assembled a mixture of hoops and tubes (Fig.
The diagnosis of each patient is shown in Table 1. Tissue samples were homogenized in a Dounce homogenizer on ice in 1 ml of lysis buffer (25 mM MES, 1 mM MgSO 4 , 2 mM EGTA, 0.1 mM GTP, 0.1% Triton X-100 with a protease inhibitor cocktail [Complete Protease Inhibitor Tablets; Boehringer Mannheim]).
D45 is close to and probably contacts the γ-phosphate of the GTP, and was the most severe of this group of mutations.
The internal (pipette) solution contained: 120 N-methyl-D-glucamine (NMG) MS, 20 TEA, 11 EGTA, 10 HEPES, 10 sucrose, 1 CaCl 2 , 4 MgATP, 0.1 Na 2 GTP, and 14 tris creatine phosphate, pH 7.2, osmolality ~300 mOsm kg -1.
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