Example sentences for: gtp

How can you use “gtp” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • GTP (50 μM) was added to the remaining reaction (5 μl total volume) to start the splicing reaction.

  • To terminate the reaction, 3 μl of the reaction mixture was withdrawn every three minutes and mixed with 7 μl of 10 mM EDTA in 50 mM Tris-HCl, pH 8.0 (this is an important modification of our previous protocol, as the EDTA stops GTP hydrolysis).

  • Briefly, the reaction was started by adding 3 μl of 10 mM GTP (Sigma) and 5 μCi {α- 32P} GTP (3000 Ci/mmol, Amersham) to 27 μl of FtsZ in MEMK6.

  • Several laboratories have shown that in addition to mutations in the N(T)KxD motif, which reduce overall nucleotide binding, dominant-negative Rab constructs can be generated by introducing amino acid substitutions ( e.g. , asparagine) at the position cognate to Ras S17 ( i.e. , S22 in Rab24) [ 31 40 41 42 ] . Such proteins are thought to be locked in an inactive GDP-bound state because the mutation markedly reduces their affinity for GTP, compared to GDP [ 42 ] . As shown in Fig.

  • NO is released by a cell through the action of the nitric oxide synthase (NOS) on its substrate, the amino acid arginine [ 20 ] . NO diffuses through the neighboring cells and elicits its functions by activating a soluble guanylyl cyclase (SGC) that synthesizes cGMP using GTP as a substrate [ 16 18 ] . The animal SGCs are dimeric enzymes comprised of two homologous subunits, α and β.


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