Example sentences for: gtp

How can you use “gtp” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The transcription reaction mixtures contained lug of linearized DNA; 0.5 mM(each) ATP, CTP, and UTP; 0.1 mM GTP; 0.5 mM cap analog (NEBL); 10 mM DTT; 40 U of Rnasin (Promega); 30 U of SP6 RNA polymerase; and 1 × SP6 RNA polymerase buffer (Promega) in a volume of 30 ul.

  • non hydrolysable GTP analogs and SKF96365) might also block the effects of mefloquine.

  • After sonication with a Sonic Dismembrator Model 300 with microtip (Fisher), 100 μl of the mixture was added to 50 μl of 3 × reaction buffer containing 150 mM Tris-HCl, pH 8.0; 30 mM thymidine; 3 mM DTT; 5 mM MgCl 2 , 0.3 mM GTP.

  • This has lead to the hypothesis that small GTP binding proteins act sequentially, in the GTPase cascade Cdc42→Rac→Rho [ 35 ] . Facilitating the study of Rho in regulation of cell activity has been the use of C3 exoenzyme, a toxin from Clostridium botulinum that ADP-ribosylates [ 15 16 ] and inactivates Rho proteins.

  • A recent paper has proposed that hydrolysis of GTP results in a substantial movement of the T3 loop, and suggested that this movement might be involved in the transition to the curved conformation [ 26].


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