Words similar to gst-rankl
Example sentences for: gst-rankl
How can you use “gst-rankl” in a sentence? Here are some example sentences to help you improve your vocabulary:
RAW cell osteoclasts were generated by plating exactly 5000 RAW cells per well in a 96-well dish with 200 ng/ml GST-RANKL or additional cytokines and culturing for 4-5 days.
Large, multinucleated, TRAP +osteoclasts were generated by further addition of 200 ng/ml GST-RANKL and culturing for 4-6 days.
Purification of GST-RANKL
GST-RANKL was purified as described previously [ 32 ] . Briefly, a fragment of murine RANKL cDNA was cloned inframe into the pGEX-4T vector (Amersham Pharmacia, Piscataway, USA) and expressed in BL21 bacteria (Amersham Pharmacia) induced with 0.1 mM IPTG (Gibco) for 5 hours at 30°C.
RAW cells were plated in 96-well plates, and were stimulated with GST-RANKL (200 ng/ml) and/or IFN-γ (10 ng/ml).