Words similar to glass-fiber

Example sentences for: glass-fiber

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PEDF binding to cell-surface receptors was assayed using biologically active radioligand 125I-PEDF and Y-79 cells [ 18 ] by a widely-used method with a mechanism of retention of receptors on polyethylenimine-treated glass-fiber filters based mainly on ionic interactions [ 32 ] . Polyethylenimine binds strongly to glass, which is negatively charged and integral membrane proteins tend to be acidic.
Similar results were obtained when the cell-bound 125I-PEDF was separated by centrifugation, rather than filtration through glass-fiber filters, and comparing reactions with CM versus those with 1% BSA in PBS (data not shown).
The free and bound 125I-PEDF were separated by filtration through glass-fiber filters and the bound radioactivity was determined in the filters using a β-scintillation counter (Beckman, model LS 3801).
Note that with the binding method used, the amount of 125I-PEDF retained in CM without cells is the same to the amount of non-specific 125I-PEDF binding (reactions with cells and in the presence of 50-fold molar excess of unlabeled PEDF) [ 18 ] , indicating retention by PEI-treated glass-fiber filters of PEDF-receptor or GAG-PEDF-receptor complexes rather than PEDF-GAG.