Example sentences for: germline

How can you use “germline” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Turcot's syndrome is caused by germline mutations in the gene APC , a member of the Wnt signaling pathway.

  • Of the sixteen U3 gene trap proviruses selected in ES cells that we have introduced into the germline, six resulted in obvious phenotypes (typically embryonic death) when bred to homozygosity [ 10 21 28 29 30 31 32 ] . In cases where no obvious phenotypes are observed, it is important to determine if the insert did not disrupt gene expression or if the gene is dispensable.

  • Finally, approximately 40% of inserts selected in ES cells result in obvious phenotypes following transmission into the mouse germline [ 27 28 29 ] . In the four cases examined, the virus appeared to induce null mutations [ 10 30 31 32 ] .

  • Following pair-wise mating (conjugation), each exconjugant replaces its old parental MAC with a new MAC, developed from a mitotic daughter of its genotypically new zygotic nucleus (review: [ 14 ] ). The mitotic sister of the MAC precursor (anlage) is set aside as the new diploid germline MIC, which is then heterochromatically silenced; all known gene expression is from the MAC or MAC anlage [ 15 16 17 ] . Once the anlage begins differentiation, the old MAC is apoptotically destroyed [ 18 ] . The mature exconjugant resumes feeding and establishes a vegetative clone (a karyonide) propagated by binary fission, in which the MIC replicates as a typical diploid mitotic nucleus and the MAC replicates amitotically.

  • Germline mutations in the breast and ovarian cancer susceptibility gene BRCA1 , which is located on chromosome 17q21, are associated with a predisposition to the development of cancer in these organs [ 1, 2].


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