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The luciferase assay data were generated by a benchtop scintillation counter (Topcounter, Packard) [ 10 18 ] . The Topcounter generates a file for each collection point that contains all the samples evaluated at that time; our code reorganizes this original data file such that a distinct record is retrieved for each sample (individual fly or tissue specimen dissected from it) with values listed in the order of occurrence from the beginning until the end of each experiment; the values are stored internally as a matrix whose columns represent individual subjects and whose rows represent observations at equally separated time frames.
Yeast and bacterial artificial chromosome (YAC and BAC) contigs have been generated and a positional cloning approach to identify the CFEOM1 causative gene is ongoing.
There are two homologous type IA topoisomerases present in E. coli . Topoisomerase III has potent DNA decatenating activity for resolution of plasmid DNA replication intermediates, but much weaker relaxation activity than topoisomerase I [ 17 ] . To exhibit maximal relaxation activity, topoisomerase III requires high temperature (52°C) along with low magnesium and monovalent ion [ 17 18 ] . In contrast, E. coli topoisomerase I was not active in the in vitro assay for resolution of plasmid DNA replication intermediates [ 19 ] . Removal of the C-terminal 49 amino acids from the 653 amino acid topoisomerase III protein resulted in drastic reduction of catalytic activity [ 20 ] . Fusion of the carboxyl-terminal 312 amino acid residues of E. coli topoisomerase I, which includes the entire ZD domain, onto the 605 N-terminal amino acids of topoisomerase III generated a hybrid topoisomerase that has relaxation activity resembling topoisomerase III along with weak decatenating activity [ 21 ] . Although preferring single-stranded DNA as binding substrate, topoisomerase I had been shown to also bind double-stranded DNA [ 22 ] , but there is no data available to indicate which domain in the enzyme is responsible for this interaction.
For each gene examined, we generated standard curves of input RNA and cycle number to determine the optimum cycle number within the linear range for PCR amplification (data not shown); for all genes examined, this was determined to be between 25-30 cycles.
To determine whether the ALM migration defect was due to the presence of a specific DNA sequence in the extrachromosomal arrays, we generated mec-3gfp deletion constructs containing various portions of the mec-3 upstream sequences.
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