Example sentences for: gels

How can you use “gels” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • This was accomplished by subjecting cell lysates to electrophoresis in agarose gels, followed by blotting to nitrocellulose membranes.

  • When apoptosis was evaluated, it was found to increase significantly in all fibroblast types within attached gels by comparison to the input fibroblasts (Fig.

  • The equivalent of 1.5 μg total lysate protein was loaded onto each lane of 10% SDS-PAGE gels, separated by electrophoresis, and transferred to nitrocellulose membranes using a Semi-Dry Trans-Blot apparatus (Bio-Rad).

  • Protein 10 μg (eNOS) or 80 μg (nNOS and iNOS) were separated by SDS-PAGE under reducing conditions (1%β-mercaptoethanol) using 8-16% gradient gels (Invitrogen, Carlsbad, CA, USA).

  • Electrophoresis was performed in SDS-polyacrylamide gels [ 35 ] using the indicated amount of total cell protein [ 36 ] . After electrophoresis, the proteins were transferred to nitrocellulose paper in buffer containing 25 mM Tris-HCl, 192 mM glycine, 20% v/v methanol, and 0.01% SDS, pH 8.5 [ 37 ] . Residual protein binding sites on the nitrocellulose were blocked by incubation for 30 minutes in TBST (25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 0.5% Tween-20) containing 4% non-fat dry milk.


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