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Example sentences for: gaithersburg

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2% agarose; Gibco BRL, Gaithersburg, MD, USA) of the resulting fragments revealed either the A1 homozygote (459 bp), the A2 homozygote (335 and 124 bp), or the A1/A2 heterozygote (459, 335, and 124 bp).
Cell culture media, supplements, phosphate-buffered saline (PBS, 10 X) and sera were obtained from Life Technologies (Gaithersburg, MD) unless stated otherwise.
In one such instance on July 20, Hanjour-likely accompanied by Hazmi-rented a plane from Caldwell and took a practice flight from Fairfield to Gaithersburg, Maryland, a route that would have allowed them to fly near Washington, D.C.
For first strand synthesis, RNA (200 ng) in 9 μl water was denatured at 65°C for 4 min, rapidly cooled on ice, and then resuspended in a 20 μl final volume containing 50 mM Tris-HCl pH 8.3, 75 mM KCl, 3 mM MgCl 2 , 10 mM DTT, 1 mM of each dNTP, 20 mM random hexamers and 200 U Superscript II RNase H -RT (GIBCO BRL, Gaithersburg, MD, USA).
Tissue culture medium, Lipofectin ®and TRIzol ®were purchased from Life Technologies (Gaithersburg, MD).