Words similar to gaithersburg
Example sentences for: gaithersburg
How can you use “gaithersburg” in a sentence? Here are some example sentences to help you improve your vocabulary:
with 20 μg anti-DNP-IgE (IgE hybridoma = H1 DNP-ε-26) [ 16 ] dissolved in 200 μl HMEM (Gibco-BRL, Gaithersburg, MD) with PIPES buffer (0.
The synovial tissue was minced and incubated with 0.3 mg/ml collagenase (Sigma, St Louis, MO, USA) for 1h at 37°C in RPMI 1640 medium (Life Technologies, Gaithersburg, MD, USA).
After 2 weeks of selection in 800 μg/ml of geneticin-G418 (Gibco/Life Technologies, Gaithersburg, MD, USA), BG-1 G418-resistant colonies were pooled, or individually isolated, and assayed for growth in the presence or absence of supplemented estrogen.
Sub-confluent (80-90%) LNCaP cells propagated in T25 flasks were washed with 5 ml of plain RPMI 1640 medium and trypsinized with 5 ml trypsin/EDTA solution (GIBCO-BRL, Gaithersburg, MD).
Primary antibodies were detected with peroxidase-conjugated anti-mouse or rabbit IgG secondary antibodies (KPL, Gaithersburg, MD) diluted 1:2000.
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