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Example sentences for: gaithersburg

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Total RNA from tomato leaves (5-15 mm), fruits (3-8 mm in diameter), whole flowers, and light-grown and dark-grown tomato seedlings was isolated with the Trizol Reagent from Gibco BRL (Gaithersburg, MD).
Primary antibodies were detected with peroxidase-conjugated anti-mouse or rabbit IgG secondary antibodies (KPL, Gaithersburg, MD) diluted 1:2000.
HRP was quantitated using a kit from Kirkegaard & Perry Laboratories, Inc., Gaithersburg, MD.
All enzyme reactions were performed as recommended by the manufacturers (GIBCO Bethesda Research Laboratories, Inc., Gaithersburg, Md., New England Biolabs, Beverly, Mass.)
Hypothalamic proopiomelanocortin (POMC) and agouti-related peptide (AGRP) mRNA and pituitary POMC mRNA were measured by Northern blot analysis as previously described [ 6 ] . Briefly, total RNA was extracted in TRIzol (GIBCO BRL, Gaithersburg, MD) and three micrograms of total RNA from hypothalamus, estimated by spectrophotometer and verified by gel electrophoresis, was subjected to Northern blot analysis to measure POMC and AGRP mRNA.