Words similar to fugene
Example sentences for: fugene
How can you use “fugene” in a sentence? Here are some example sentences to help you improve your vocabulary:
Transient transfections of β-catenin, β-galactosidase and Wnt reporter plasmids were performed using the Fugene reagent 24 hours after initial plating (Roche Molecular Biochemicals).
Briefly, approximately 10 8cells were transfected with pcDNA 3.1 constructs (Invitrogen) bearing either murine Smo (wild-type or W539L mutant), GFP, rat β2AR (Invitrogen), or murine Ptc cDNAs using Fugene 6 (Roche).
In transient transfection experiments, optimal amounts of these DNA were transfected into monolayers-grown cells using the Fugene 6 reagent (Roche Diagnostics, Indianapolis, IN).
A stable BS-C-1 cell line was made by transfection of GFP-Arp3 (gift from D. Schafer as described [ 34 ] ) using Fugene6 (Roche) and selection with 500 ug/ml Geneticin.
Transfections were carried out using the Fugene reagent, according to the manufacturer's instructions.
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