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An alignment output from this source was used to create a dendrogram using TreeTop [ 45 ] with PHYLIP output (default parameters) at a node of the European Molecular Biology Network http://www.genebee.msu.su [ 47 ] . Potential phosphorylation sites were identified using NetPhos 2.0 http://www.cbs.dtu.dk/services/NetPhos [ 48 ] . After alignment with CLUSTAL W, identical and similar amino acids in Figure 3were identified using MacBoxshade 2.15 http://www.isrec.isb-sib.ch/ftp-server/boxshade/MacBoxshade.
The strings served as an input to the Perl script prosite_scan (author: Kay Hofmann http://www.isrec.isb-sib.ch/ftp-server/prosite_scan/pattern_find) searching the nucleotide section of GenBank (downloaded April 12 th2000).