Words similar to fs-α
Example sentences for: fs-α
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The skeletal nature of the EC coupling expressed by fs-α 1S is shown in Fig.
This result is consistent with the mobility of the native purified skeletal muscle α 1S subunit [ 25 ] . The fs-α 1S migrated with a molecular weight of approximately 90 KDa which is entirely consistent with the theoretical molecular weight of the expressed fragment which was 85.
Thus we are certain that the EC coupling expressed by fs-α 1S is mechanistically similar to control skeletal-type EC coupling but limited in magnitude by a comparatively lower density of functional DHPRs that are assembled in cells expressing fs-α 1S . It is conceivable that the functional integrity of the fragmented α 1S protein is maintained in part by the β subunit of the DHPR which spans both halves of the α1 pore subunit by binding to the I-II loop and the C-terminus [ 35 36 ] . Consistent with this explanation, we failed to detect EC coupling recovery when fs-α 1S was expressed in β1-null myotubes.
The mechanism of translation of the fs-α 1S clearly deserves closer scrutiny in the future.
However, re-initiation after a stop has only been described for cases in which the leader ORF is no longer than 30 codons because initiation factors fall off shortly after recognition of the initiator AUG [ 32 ] . In the case of fs-α 1S , what can be considered the "leader" ORF encodes for a protein of 671 residues.