Words similar to fs-α
Example sentences for: fs-α
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This indicated that the absence of EC coupling observed in myotubes expressing fs-α 1S M701I was not due the production of a labile protein.
Hence the signaling mechanism, like that reported in normal myotubes and dysgenic myotubes expressing wt-α 1S , was Ca 2+entry independent [ 29 ] . We also expressed fs-α 1S in cultured myotubes from two available gene knock-out (KO) mice, lacking the endogenous β1a isoform of the skeletal muscle DHPR [ 30 ] and lacking RyR1 [ 31 ] . As shown in Fig.
fs-α 1S did not express L-type Ca 2+current even though it was consistently able to activate the Ca 2+transient in 15 of 15 cells.
6C) favor the explanation that 1) fs-α 1S recovers DHPR function by virtue of expressing two complementary fragments of α 1S and 2) the expression of the C-terminal half of α 1S by fs-α 1S is likely to occur by leaky ribosomal scanning.
Primers for the frame-shift mutant, fs-α 1S , were originally designed to delete the 20-mer Thr671-Leu690 in the cytosolic loop between repeats II and III of α 1S and to generate a full-length α 1S carrying this internal deletion.