Words similar to fs-α
Example sentences for: fs-α
How can you use “fs-α” in a sentence? Here are some example sentences to help you improve your vocabulary:
This result is consistent with the mobility of the native purified skeletal muscle α 1S subunit [ 25 ] . The fs-α 1S migrated with a molecular weight of approximately 90 KDa which is entirely consistent with the theoretical molecular weight of the expressed fragment which was 85.
Thus we are certain that the EC coupling expressed by fs-α 1S is mechanistically similar to control skeletal-type EC coupling but limited in magnitude by a comparatively lower density of functional DHPRs that are assembled in cells expressing fs-α 1S . It is conceivable that the functional integrity of the fragmented α 1S protein is maintained in part by the β subunit of the DHPR which spans both halves of the α1 pore subunit by binding to the I-II loop and the C-terminus [ 35 36 ] . Consistent with this explanation, we failed to detect EC coupling recovery when fs-α 1S was expressed in β1-null myotubes.
Except for the magnitude, the SR Ca 2+release signal expressed by fs-α 1S was entirely typical of skeletal myotubes with sigmoidal voltage-dependence, proceeding in the absence of external Ca 2+and requiring RyR1.
Cells were fixed and processed for immunofluorescence as described [ 4 20 ] . The N-terminal fragment expressed by fs-α 1S or wt-α 1S was identified with a mouse monoclonal antibody against a T7 epitope fused to the N-terminus of α 1S . The anti-T7 antibody (Novagen, WI) was used at a dilution of 1:1000.
Thus, we are confident that the voltage-evoked Ca 2+transient in cells transfected by fs-α 1S cells was a direct consequence of the expressed protein.
Loading...