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Example sentences for: fruitfly
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These include 1) the strength of the 3' and 5' splice sites, which can be calculated using the consensus values that were determined by Shapiro and Senepathy [ 5 ] or by the Splice Site Prediction program by Neural Networks (SSPNN, http://www.fruitfly.org/seq_tools/splice.html) which takes more known sequences into account, 2) the branch point sequences and 3) certain sequences such as exonic splicing enhancers (ESE) that can activate the use of a weak 3' splice site or elements such as exonic splicing silencer elements (ESS) that inhibit splicing [ 1 ] . In addition to these cis -acting elements, exon length [ 6 ] and secondary structure have also been mentioned to play a role in splicing [ 7 ] .
Calculations were performed using the S plice S ite P rediction by N eural N etwork (SSPNN, http://www.fruitfly.org/seq_tools/splice.html) program (Table 1).
The top row comes from a healthy, robust individual wild-type fruitfly; the bottom row is a record from another individual, whose activity dwindled.
The sequence environment of all donor- and acceptor sites was analysed using Splice Site Prediction by Neural Network (SSPNN, URL address: http://www.fruitfly.org/seq_tools/splice.html).
Sequence retrieval was initiated via BLASTN searches (default parameters- [ 37 ] ) against the BDGP http://www.fruitfly.organd GenBank http://www.ncbi.nlm.nih.govdatabases using LTRs from previously identified Drosophila retroelements as queries [ 15 ] . Results with E-values < e -10were annotated on the corresponding clone, whereupon visual inspection of several annotations confirmed the presence of retroelements proximal to known genes.
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