Words similar to freeze-thaw

Example sentences for: freeze-thaw

How can you use “freeze-thaw” in a sentence? Here are some example sentences to help you improve your vocabulary:

Cells were then resuspended in buffer A (50 mM Tris-HCl pH 7.5, 20% glycerol, 1 mM Dithiothreitol, 0.2% Nonidet P-40) with 0. 5 M KCl and subjected to two rounds of freeze-thaw followed by a 30 min incubation on ice.
Tat and gp120 were highly susceptible to degradation and loss of biological activity with each freeze-thaw cycle.
Control and treated cells were lysed by repeated freeze-thaw cycles with buffer containing 10 mM HEPES (pH 7.5), 1.5 mM Mg(OAc) 2 , 1 mM dithiothreitol (DTT), 0.5% NP40, 5% glycerol, 0.5 mM phenylmethylsulfonyl fluoride, and 10 μg/ml each of the protease inhibitors aprotinin, pepstatin, and leupeptin.
Following 5 freeze-thaw cycles, the cell suspensions were subjected to centrifugation for 5 min at 5000 rpm at 4°C in an Eppendorf 5417R centrifuge.
Briefly, cells were suspended in 50 mM Tris, pH 8.0, 100 mM NaCl, 1 mM EDTA, 1 mM PMSF, treated with 0.4 mg/mL lysozyme and lysed by freeze-thaw and sonication.