Words similar to formazan
Example sentences for: formazan
How can you use “formazan” in a sentence? Here are some example sentences to help you improve your vocabulary:
The formazan product was solubilized by addition of 100 μl of dimethyl sulfoxide (DMSO) and 100 μl of 10% SDS (in 0.01 M HCl) for 16 hours at 37°C.
Plates were incubated in a humidified 5% CO 2 incubator at 36°C and the reduction of the WST-1 reagent to formazan was measured repeatedly after the addition of the WST-1 reagent, at either 415 nM (neurons) or 450 nM (microglia), with a microtiter plate (ELISA) reader.
The assay is based upon the cleavage of the yellow tetrazolium salt MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] to purple formazan crystals by metabolically active cells [ 15 ] . The tested concentration of Gleevec ranged from 0.5 to 8 μM.
The dehydrogenase activity was expressed as the absorbance (read with a Molecular Devices microplate reader) of the formazan product at 575 nm.
The formazan accumulated intracellularly and could be visualized by epifluorescence microscopy in wet-mount preparations, on polycarbonate membrane filter surfaces, or directly in some biofilms.
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