Example sentences for: formazan

How can you use “formazan” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The assay is based upon the cleavage of the yellow tetrazolium salt MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] to purple formazan crystals by metabolically active cells [ 15 ] . The tested concentration of Gleevec ranged from 0.5 to 8 μM.

  • The purple formazan product indicative of viable cells was then dissolved by adding 250 μl acidified isopropanol (95% isopropanol; 5% 2N HCl), and 200 μl aliquots were collected.

  • The wavelength to measure absorbance of formazan product is 570 nm, with a reference wavelength of 750 nm.

  • Plates were incubated in a humidified 5% CO 2 incubator at 36°C and the reduction of the WST-1 reagent to formazan was measured repeatedly after the addition of the WST-1 reagent, at either 415 nM (neurons) or 450 nM (microglia), with a microtiter plate (ELISA) reader.

  • A colorimetric assay for the quantitation of cell viability, based on the cleavage of the tetrazolium salt WST-1 (4-[3-(4-Iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]1,3-benzene disulfonate) (slightly red) to formazan (red) by mitochondrial dehydrogenases in viable cells was utilized for these studies.


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