Words similar to formazan
Example sentences for: formazan
How can you use “formazan” in a sentence? Here are some example sentences to help you improve your vocabulary:
The assay is based upon the cleavage of the yellow tetrazolium salt MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] to purple formazan crystals by metabolically active cells [ 15 ] . The tested concentration of Gleevec ranged from 0.5 to 8 μM.
A colorimetric assay for the quantitation of cell viability, based on the cleavage of the tetrazolium salt WST-1 (4-[3-(4-Iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]1,3-benzene disulfonate) (slightly red) to formazan (red) by mitochondrial dehydrogenases in viable cells was utilized for these studies.
Plates were incubated in a humidified 5% CO 2 incubator at 36°C and the reduction of the WST-1 reagent to formazan was measured repeatedly after the addition of the WST-1 reagent, at either 415 nM (neurons) or 450 nM (microglia), with a microtiter plate (ELISA) reader.
The dehydrogenase activity was expressed as the absorbance (read with a Molecular Devices microplate reader) of the formazan product at 575 nm.
The wavelength to measure absorbance of formazan product is 570 nm, with a reference wavelength of 750 nm.