Example sentences for: formazan

How can you use “formazan” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • A colorimetric assay for the quantitation of cell viability, based on the cleavage of the tetrazolium salt WST-1 (4-[3-(4-Iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]1,3-benzene disulfonate) (slightly red) to formazan (red) by mitochondrial dehydrogenases in viable cells was utilized for these studies.

  • This assay for cell viability is based on the reduction of 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) by mitochondrial dehydrogenase in viable cells to produce a purple formazan product.

  • The dehydrogenase activity was expressed as the absorbance (read with a Molecular Devices microplate reader) of the formazan product at 575 nm.

  • The wavelength to measure absorbance of formazan product is 570 nm, with a reference wavelength of 750 nm.

  • On the 6 thday, XTT dye (1 mg/ml) was added to the cells, Intracellular formazan was determined spectrophotometrically [ 18 19 ] . Similar experiments were done with HIV-1 BaL, except that PM1 cells were used instead of MT-2 cells, and virus production was measured by ELISA for p24 antigen one week after infection.


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