Words similar to formazan
Example sentences for: formazan
How can you use “formazan” in a sentence? Here are some example sentences to help you improve your vocabulary:
The formazan product was solubilized by addition of 100 μl of dimethyl sulfoxide (DMSO) and 100 μl of 10% SDS (in 0.01 M HCl) for 16 hours at 37°C.
A colorimetric assay for the quantitation of cell viability, based on the cleavage of the tetrazolium salt WST-1 (4-[3-(4-Iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]1,3-benzene disulfonate) (slightly red) to formazan (red) by mitochondrial dehydrogenases in viable cells was utilized for these studies.
This assay for cell viability is based on the reduction of 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) by mitochondrial dehydrogenase in viable cells to produce a purple formazan product.
Plates were incubated in a humidified 5% CO 2 incubator at 36°C and the reduction of the WST-1 reagent to formazan was measured repeatedly after the addition of the WST-1 reagent, at either 415 nM (neurons) or 450 nM (microglia), with a microtiter plate (ELISA) reader.
The dehydrogenase activity was expressed as the absorbance (read with a Molecular Devices microplate reader) of the formazan product at 575 nm.