Example sentences for: formamide

How can you use “formamide” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After an overnight hybridization, the embryos were washed at high stringency in prewarmed 50% formamide, 5X SSC, 1% SDS (wash I) at 70°C for 90 minutes.

  • Resuspended probes were mixed with 4 μl of 5 × hybridization buffer (20 × SSX, 1%SDS), 1 μl human Cot-1 DNA (10 μg/μl), 1 μl polyA DNA (2 μg/μl) (Amersham) and 8 μl formamide (Sigma, St. Louis, MO), heated to 65°C for 2 min and then centrifuged briefly.

  • Re-hydrated and post-fixed embryos were incubated for 1 h in hybridization buffer (50% formamide, 4× SSC, and 0.01% Tween 20).

  • 05% Tween 20 at 37°C -55°C with or without 50% - 60% formamide.

  • The pellets were washed twice with 100 μl of 70% ethanol, air dried and dissolved in 25 μl of molecular biology grade formamide.


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