Words similar to formamide
Example sentences for: formamide
How can you use “formamide” in a sentence? Here are some example sentences to help you improve your vocabulary:
The probe DNA mixtures were ethanol precipitated and dissolved in hybridization buffer (50 % formamide, 2 × SSC, 10 % dextran sulphate).
Early studies with high density arrays suggested the use of 25 mer in situ synthesized oligodeoxyribonucleotides [ 1 ] . A more recent study using covalently attached oligodeoxyribonucleotides found that 30- and 35 mer oligodeoxyribonucleotides generated signals two- to five-fold higher than 25 mers, and signals obtained form 60 mers were only two-fold higher than those obtained from 30 mers but ten-fold higher than those obtained from 25 mers [ 24 ] . In fact, in situ synthesized 60 mer oligodeoxyribonucleotides, hybridized in 30-32% formamide, have been shown to represent a compromise between maximal sensitivity and specificity compared to other lengths and hybridization conditions [ 12 ] . Lastly, a study employing
Blots were then prehybridized overnight at 42°C in 50% formamide, 4X SSC, 5X Denhart's solution, 25 mM sodium phosphate, pH 6.5, 1.0% sodium deodecyi sulphate (SDS), and 200 μg/ml salmon sperm DNA.
The probe mixtures were then applied to rat fibroblast metaphase chromosome slides (previously denatured at 73°C for 2 min in 70 % formamide, 2 × SSC).
8 and that it was preferable to adjust pH hybridization mix (buffer, formamide, dextran sulfate) directly.