Words similar to formamide

Example sentences for: formamide

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The DNA on the microarray was then subjected to a UV-cross-linking at a dose of 150 mJ/cm 2. Prehybridization was performed by incubating the slide in a fresh mixture of 100 ml of 25% formamide, 5X SSC, 0.1% SDS, and 1% BSA for 45 min. at 42°C in a Coplin jar.
Gentle vacuum was used to remove the hybridization solution and the embryos were subjected to eight 30-min washes in wash buffer (50% formamide, 2× SSC and 0.01% Tween 20).
The sections were then prehybridized for 2 hr at 58°C in the hybridization mix (50% formamide, 5×SSC, salmon sperm DNA 40 μg/μl; 500 μl on each section).
Pellets were resuspended in 50 μl of 50% formamide, 5 mM Tris-HCl pH 7.5, 0.5 mM EDTA and 0.01% Tween 20.
The probe mixtures were then applied to rat fibroblast metaphase chromosome slides (previously denatured at 73°C for 2 min in 70 % formamide, 2 × SSC).