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Example sentences for: formamide

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The melting temperature T m is determined by a number of factors that include temperature, monovalent cation concentration, formamide, pH, probe fragment length, GC content and mismatched hybrids.
Hybridization was performed overnight at 67°C in a sealed bag with 125 μl/sq cm fresh hybridization solution containing formamide and salmon sperm DNA as above with the addition of 40 ng/ml digoxigenin labeled probe denatured by boiling for 5 minutes.
Reactions were stopped by addition of loading buffer: 80% formamide, 10 mM Na 2 EDTA (pH 8.0), and 1 mg/ml each bromophenol blue and xylene cyanol.
Prior to genotyping, the double stranded polonies were made single stranded by stripping away the non-acrydited strand in a two-step procedure [ 3 ] . The polony DNA was denatured by incubation in a formamide buffer (1x SSC, 70% formamide, 25% doubly deionized-water) at 70 C for 15 min.
For those BACs that demonstrated significant cross-hybridizaton to other chromosomal regions, FISH was repeated using higher stringency (60% formamide) hybridization solution and post-hybridization washes.