Example sentences for: formamide

How can you use “formamide” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The process of formamide denaturation, hybridization, extension, and scanning was repeated 3 additional times for each primer in order to do a single base extension with each of the four labeled nucleotides.

  • The blots were pre-hybridized in a solution containing 6-strength SSC (150 mM NaCl, 15 mM Na-citrate, pH 7.0), 0.5% SDS, 50% formamide, and 100 mg/ml denatured sperm DNA at 42°C for 2-4 h, and were hybridized overnight in the same buffer containing approximately 10 6cpm/ml of radiolabeled cDNA probe.

  • 75 μl using SpeedVac and remainder of the hybridization components containing 2.5 μl of 10 μg/μl salmon sperm DNA, 8.75 μl 20X SSC, 0.25 μl 10% SDS, and 8.75 μl formamide were added.

  • To remove excess dye terminators, the sequencing product is purified by ethanol precipitation and resuspended in 10 μL of HiDi formamide.

  • Hybridization was performed in the presence of 50% formamide at 42°C for 16 hours.


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