Words similar to formaldehyde
Example sentences for: formaldehyde
How can you use “formaldehyde” in a sentence? Here are some example sentences to help you improve your vocabulary:
Total RNA (20 μg) was fractionated on 1% agarose gels containing formaldehyde and transferred to DURLON-UV (Stratagene) nylon membranes.
7% formaldehyde at -20°C for 20 minutes, then washed twice with 1 × PBS; the coverslips where then incubated with a 1:200 dilution of rabbit anti-cyclin D 1 antibody (Santa Cruz Biotechnology) or control rabbit sera (Biogenex) for 2 hours at room temperature followed by three washes with 1 × PBS; the coverslips were then incubated with a 1:250 dilution of rhodamine-labeled donkey-anti-rabbit anti-sera (Chemicon) for 45 minutes to detect the primary antibody; following three final washes with 1 × PBS, the coverslips were mounted on glass slides using ProLong Antifade (Molecular Probes) for fluorescent detection of GFP and cyclin D 1 on an Olympus IX70 microscope at 100 × magnification.
Cells grown on coverslips were treated with H 2 O 2 for various periods of time as indicated in figure legends, fixed for 10 minutes with 3.7% formaldehyde (prepared in PBS), stained with 1 μg/ml bisbenzimide solution (Hoechst 33258, Sigma, St. Louis, MO) for 10 minutes at room temperature, mounted with GEL/MOUNT, then observed under a fluorescent microscope as described in the previous section.
2 μm, Schleicher and Schuell) in triplicate in denaturing solution (66 % formamide, 7.9 % formaldehyde, 26 mM MOPS buffer, pH 7.0, 1.3 mM EDTA, 0.066 M sodium acetate) using a slot blot apparatus.
We examined the effect of incubation time and temperature during fixation with 4% PFA in PBS(+), since conditions for irreversible crosslinking of polypeptides in cultured cells with formaldehyde are unknown.
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