Words similar to fnr

Example sentences for: fnr

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To introduce mutations within or nearby the proposed Fnr recognition site at the dmsA promoter, site-directed mutagenesis was performed using the method of Kunkel [ 33 ] . The template for mutagenesis was m13mp19-100 which contained a 676 bp Bam HI fragment containing 587 bp of DNA upstream of the dmsA translational start site and the associated 89 bp of the dmsA coding region.
Several additional DNA sequence changes were introduced into the dmsA promoter region to alter the Fnr recognition sequence as well as to alter the spacing between the Fnr site and the RNA polymerase recognition sequences (Figure 1).
The λJA303 consensus mutant fnr +strain exhibited a 1000-fold anaerobic increase in β-galactosidase expression relative to the corresponding fnr -strain (i.e.
The data illustrates that Fnr is responsible for the 100-fold anaerobic activation of dmsA expression.
This is in contrast to the requirement for the left half-site of the Fnr1 regulatory site needed for Fnr-dependent repression at the cydA P1 promoter [ 15 ] . When a two base-pair change was introduced into the left Fnr half-site (e.g.