Words similar to fluoroorotic
Example sentences for: fluoroorotic
How can you use “fluoroorotic” in a sentence? Here are some example sentences to help you improve your vocabulary:
Strains lacking the wild type SEC/URA3/CEN plasmids were isolated by growth on media containing 5-fluoroorotic acid (5-FOA).
ARS , autonomously replicating sequence; 5-FOA, 5-fluoroorotic acid; TAR, transformation-associated recombination; YAC, yeast artificial chromosome.
Optimum conditions for TAR cloning have been recently described [ 30 ] . Transformants were selected on synthetic histidine-minus plates containing 5-fluoroorotic acid (5-FOA) to select clones that no longer express URA3 [ 31 ] . Because of its toxic effect on yeast spheroplasts, 5-FOA was not included in the top agar.
One to two colonies were picked from each plate and tested for viability on SD+ 5-fluoroorotic acid (5-FOA) plates [ 58 ] . When more than one colony was picked from a single plate, care was taken to pick colonies of different size or morphology.
A screen of a HeLa cell library yielded 56 colonies that tested positive in the two hybrid assays (growth on plates lacking histidine or uracil, lack of growth on plates containing 5-fluoroorotic acid; and induction of lacZ resulting in blue color when assayed with X-gal).