Words similar to fls

Example sentences for: fls

How can you use “fls” in a sentence? Here are some example sentences to help you improve your vocabulary:

Centrifugal concentration of viral supernatant by 10- to 100-fold significantly increased the efficiency of viral transduction, with 50% or more of FLS expressing EGFP in several independent experiments using FLS lines from separate donors.
We report a retroviral vector system and transduction methodology that achieve stable transgene expression in primary human FLS with efficiencies of approximately 50%.
cfu = colony forming units; COX-2 = cyclooxygenase-2; DMEM = Dulbecco's modified Eagle's medium; EGFP = enhanced green fluorescent protein; FACS = fluorescence-activated cell sorting; FLS = fibroblast-like synovial cells; MoMLV = Moloney murine leukemia virus; PCR = polymerase chain reaction; RA = rheumatoid arthritis; RCF = relative centrifugal force.
First, our viral backbone is a modified MoMLV vector that incorporates genetic elements (myeloproliferative sarcoma virus long-terminal repeats and B2 mutation) associated with resistance to transcriptional silencing following proviral integration in primary cells [ 7 8 ] . While we did not perform a detailed comparison of EGFP expression in FLS using the modified and unmodified vector backbones, preliminary experiments suggested that the modified vector was superior (J Yang, unpublished observations).
2 ± 8.2%) of FLS with unconcentrated supernatant (titer of 10 6cfu/ml) that was roughly comparable to prior reports.