Words similar to floxed
Example sentences for: floxed
How can you use “floxed” in a sentence? Here are some example sentences to help you improve your vocabulary:
BigT was then digested with PacI and AscI to release the entire floxed neo-tpA and CFP assembly, and inserted into pROSA26PA digested with PacI and AscI.
BigT was then digested with PacI and AscI to release the entire floxed neo -tpA and EYFP assembly, and inserted into pROSA26PA digested with PacI and AscI.
The R26R allele terminates transcription prematurely, but when the mice are crossed with Cre-expressing transgenic mice, the Cre-mediated excision of the floxed termination sequence leads to constitutive lacZ expression.
However, when the reporter mice were crossed to β-actin-Cre transgenic mice [ 23], which express Cre constitutively in most or all cells including those in the early embryo, the resulting doubly transgenic embryos displayed apparently ubiquitous expression of EYFP or ECFP (Figure 2), indicating efficient excision of the floxed stop sequence.
Considering the utility of the ROSA26 locus for expressing various sequences in a ubiquitous manner [ 4, 5, 21], we generated a generic targeting vector (pBigT), into which any sequence of interest can easily be inserted downstream of a floxed neo-tpA cassette, and then subsequently cloned into a plasmid with the ROSA26 genomic flanking arms.