Example sentences for: flask

How can you use “flask” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The frozen cell pellets from 500 ml spinner flask culture were lysed by sonication in Buffer A (50 mM Tris-HCL pH 8, 5 mM β-mercaptoethanol, 1 mM EDTA, 1 μg/ml leupeptin, pepstatin, and E64, and 1 mM PMSF) containing 450 mM NaCL and 1% NP40.

  • Twenty-liter cultures of P. aeruginosa strain PAO1 (1 l per 2.8-1 flask) were grown to late-exponential phase in M-9 salts/glucose medium, and the periplasmic fraction was separated from the rest of the cells as described above, but with a scaled-up protocol.

  • One week later, the VSMCs had outgrown from the tissue suspension, and were transferred to a new flask.

  • Culture dynamics were determined for SH-SY5Y neuroblastoma cells at an initial density of 4.0 × 10 4cells/cm 2. Cells were introduced into five 25 cm 2flasks, then harvested from one flask each day using 5 ml Puck's saline solution (pH 7.3, 137 mM NaCl, 5.4 mM KCl, 0.7 mM Na 2 HPO 4 , 9.5 mM HEPES, 5.6 mM dextrose, and 0.8 mM EDTA).

  • RNA (10 μg) extracted from each individual flask was hybridized to a single gene chip (i.e.


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