Example sentences for: flask

How can you use “flask” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Isopycnic fractionation in sucrose gradients was performed essentially as described [ 43 ] . Briefly, cells (one confluent T162 flask of HT1080 or NIH 3T3 cells expressing different CD98 constructs) were lysed in 2 ml 500 mM sodium carbonate buffer, pH 11, in the presence of protease inhibitors and passed through 25 gauge needle 5 times followed by 6 passages through 27 gauge needle.

  • Culture dynamics were determined for SH-SY5Y neuroblastoma cells at an initial density of 4.0 × 10 4cells/cm 2. Cells were introduced into five 25 cm 2flasks, then harvested from one flask each day using 5 ml Puck's saline solution (pH 7.3, 137 mM NaCl, 5.4 mM KCl, 0.7 mM Na 2 HPO 4 , 9.5 mM HEPES, 5.6 mM dextrose, and 0.8 mM EDTA).

  • What law or principle--secular, humanist, or religious--says that one combination of genetic material in a flask is OK, but another is not?

  • , washed twice in a 50:50 mixture of OPTI-MEM and Plasbumin and incubated for 3 and 24 h at 1 × 10 8cells/T175 flask in 50 ml serum-free OPTI-MEM in the presence or absence of 6,000 IU IL-2 per ml OPTI-MEM.

  • The frozen cell pellets from 500 ml spinner flask culture were lysed by sonication in Buffer A (50 mM Tris-HCL pH 8, 5 mM β-mercaptoethanol, 1 mM EDTA, 1 μg/ml leupeptin, pepstatin, and E64, and 1 mM PMSF) containing 450 mM NaCL and 1% NP40.


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