Words similar to fixatives
Example sentences for: fixatives
How can you use “fixatives” in a sentence? Here are some example sentences to help you improve your vocabulary:
Crosslinking of polypeptides was also evaluated after treatment with several other fixatives that preserved microextensions well and that were found to be useful previously for moesin staining (Figure 6B).
Considering their effect on proteins, fixatives in general can be divided into two types: "coagulant" or "non-coagulant".
Only a small amount of polypeptides was extracted when cells were fixed with glutaraldehyde or DSP-Tsb (Figure 6B, lane 2, 3 and 6) and the cells were morphologically well preserved by these fixatives as observed by SEM, indicating that most proteins were irreversibly crosslinked.
Acetone also provided good preservation of microextensions even after preparing the cells for immunolabeling, whereas some of the microextensions were lost, when cells were fixed with other coagulant fixatives (Table 2).
On the other hand, when cells were fixed with PLP or LP and treated with 1% saponin in 3% BSA for permeabilization, most of the polypeptides were either not crosslinked by these relatively mild fixatives or crosslinks were reversed by the addition of SDS sample buffer (Figure 6B, lanes 4 and 5).
Loading...