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Example sentences for: fixatives

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Only a small amount of polypeptides was extracted when cells were fixed with glutaraldehyde or DSP-Tsb (Figure 6B, lane 2, 3 and 6) and the cells were morphologically well preserved by these fixatives as observed by SEM, indicating that most proteins were irreversibly crosslinked.
On the other hand, when cells were fixed with PLP or LP and treated with 1% saponin in 3% BSA for permeabilization, most of the polypeptides were either not crosslinked by these relatively mild fixatives or crosslinks were reversed by the addition of SDS sample buffer (Figure 6B, lanes 4 and 5).
Crosslinking of polypeptides was also evaluated after treatment with several other fixatives that preserved microextensions well and that were found to be useful previously for moesin staining (Figure 6B).
The coagulant fixatives, including methanol [ 8, 9], ethanol [ 10], acetone [ 11], and trichloroacetic acid [ 10, 12], denature and precipitate proteins.
We have performed ultrastructural analysis by SEM of cells fixed and/or extracted by the methods listed in Table 1. When the cells were treated with coagulant fixatives, the cytoskeleton was apparently exposed, but the three dimensional structures at the edge of the cells were not preserved as well as those prepared by the DOTMAC/PFA method (data not shown).