Words similar to fitc-conjugated
Example sentences for: fitc-conjugated
How can you use “fitc-conjugated” in a sentence? Here are some example sentences to help you improve your vocabulary:
Phenotypic characterization of T cells was done by two-color flow cytometry (Epic XL FL, Beckman Coulter, Allendale, New Jersey, United States) using combinations of the following monclonal antibodies: FITC-conjugated anti-CD4, -CD8, and -CD14; PE- and Cy5-conjugated anti-CCR4, -CCR3, -CCR5, and -CXCR3.
Cells were fixed with 4% paraformaldehyde, and indirectly immunostained with an anti-FLAG antibody (Sigma) and then a FITC-conjugated goat anti-mouse secondary antibody (Molecular Probes).
Microscopy for FITC-conjugated TTP analysis was completed using a BioRad MRC1024 laser scanning confocal microscope (BioRad, Hercules, CA, USA).
After incubation, the cells were washed in permeabilization buffer and resuspended in FITC-conjugated F(ab') 2 (antigen-binding fragment) donkey antirabbit IgG (25 μg/ml, Jackson ImmunoResearch, West Grove, PA, USA) for 45 min at 4°C.
Mean G 1 fluorescence of samples stained with the anti-Tag isotype, IgG2a, and FITC-conjugated secondary antibody served as a control for non-specific fluorescence and was subtracted from the G 1 fluorescence of corresponding Tag-stained samples (Figure 4).
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