Words similar to fitc-conjugated

Example sentences for: fitc-conjugated

How can you use “fitc-conjugated” in a sentence? Here are some example sentences to help you improve your vocabulary:

After washing three times, cells were then incubated in FITC-conjugated anti-mouse secondary antibody (1 : 400) dilution for 1 h at 37°C, followed by washing three times with anti-fade equilibrating buffer and mounting in anti-fade mounting medium (Molecular Probe, Eugene, OR, USA).
Thereafter, cells were washed three times for 5 min in PBS and incubated for 45 min at room temperature with TRITC- or FITC-conjugated anti-rabbit IgG secondary antibody (Sigma) diluted 1:75.
For the infliximab inhibition experiments, a goat polyclonal antibody to TTP was used (Santa Cruz Biotechnology Inc, Santa Cruz, CA) with a secondary swine antigoat FITC-conjugated IgG antibody (Sigma).
RA synovial tissue T cells were analyzed without in vitro culture or stimulation by staining synovium mononuclear cells with FITC-conjugated anti-CD4 mono-clonal antibody (Q4120; Sigma) and PE-conjugated anti-CD45RO monoclonal antibody (UCHL-1), after which individual CD4 +CD45RO +T cells were sorted in a 96-well PCR plate (Robbins Scientific, Sunnyvale, CA, USA) using the FACStar Plus(Becton Dickinson, San Jose, CA, USA).
The samples were rinsed quickly in PBS and then incubated for 30 minutes at 37°C in PBS containing the secondary antibodies, a FITC-conjugated goat anti-rabbit IgG at a dilution of 1:50 and a TRITC-conjugated goat anti-mouse IgG at a dilution of 1:400.