Words similar to fitc-conjugated

Example sentences for: fitc-conjugated

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Detection of hybridized fluorescein-labeled SP-B riboprobe was accomplished by reacting the sections with rabbit anti-fluorescein antibody followed by FITC-conjugated goat anti-rabbit antibody.
The samples were rinsed quickly in PBS and then incubated for 30 minutes at 37°C in PBS containing the secondary antibodies, a FITC-conjugated goat anti-rabbit IgG at a dilution of 1:50 and a TRITC-conjugated goat anti-mouse IgG at a dilution of 1:400.
Mean G 1 fluorescence of samples stained with the anti-Tag isotype, IgG2a, and FITC-conjugated secondary antibody served as a control for non-specific fluorescence and was subtracted from the G 1 fluorescence of corresponding Tag-stained samples (Figure 4).
Cells were fixed with 4% paraformaldehyde, and indirectly immunostained with an anti-FLAG antibody (Sigma) and then a FITC-conjugated goat anti-mouse secondary antibody (Molecular Probes).
The following day, the sections were rinsed again and incubated for 30 minutes at room temperature with FITC-conjugated sheep anti-mouse IgG (Boehringer-Mannheim; diluted 1:100).