Words similar to fitc
Example sentences for: fitc
How can you use “fitc” in a sentence? Here are some example sentences to help you improve your vocabulary:
Sections were then incubated for 30 min at 4°C in fluorescein isothiocynate (FITC)-conjugated anti-rabbit antibody (Sigma) diluted 1:500 in blocking solution, and then washed three times for 5 min each in 1x PBS.
There is no overlap between the excitation and emission spectra for the FITC and Cy5 secondary antibodies used in the double label experiments.
BSA = bovine serum albumin; DMEM = Dulbecco's modified Eagle's medium; FACS = fluorescence-activated cell sorting; Fc = crystallizable fragment; FITC = fluorescein isothiocyanate; IFN = interferon; IL = interleukin; MHC = major histocompatibility complex; NF = nuclear factor; NO = nitric oxide; RANK = receptor-activator of NFκB; RANKL = receptor-activator of NFκB ligand; Th = T helper cells; TRAP = tartrate-resistant acid phosphatase.
Monoclonal antibodies used in the study were: 1) mAb against human HLA-DR,DP,DQ, FITC conjugate (anti-MHCII-FITC), clone Tü39, mouse IgG2a; 2) mAb against human IFNg receptor R1 chain, biotin conjugate, clone MMHGR-1, mouse IgG1; 3) mAb against human IFNg receptor R2 chain, biotin conjugate, clone MMHGR-2, mouse IgG1; 4) mouse IgG2a isotype control mAb, FITC conjugate (IgG2a-FITC); 5) mouse IgG1 isotype control mAb, biotin conjugate; 6) mAb against Rb protein, FITC conjugate (Rb-FITC), clone G3-245, mouse IgG1; 7) mouse IgG1 isotype control mAb, FITC conjugate (IgG1-FITC).
APC = allophycocyanin; ARE = AU-rich element; CM = complete media; ELISA = enzyme-linked immunosorbent assay; FACS = fluorescence-activated cell sorting; FITC = fluorescein isothiocyanate; IL = interleukin; KLH = keyhole limpet hemocyanin; LPS = lipopolysaccharide; MESF = milliequivalents of soluble fluorescein; RPMI = Roswell Park Memorial Institute [medium]; RT-PCR = reverse transcriptase polymerase chain reaction; TNF = tumor necrosis factor; TNFR = TNF receptor; TTP = tristetraprolin; WBC = white blood cell.