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References Briggs 1988; Brown 1978; Espinosa 1985; Fisher 1958; Henderson 1937; Ingalls 1996; Rael 1950; Robb 1980
0 × 10 6cells/ml) were incubated in 24-well Falcon plates (Fisher Scientific, Pittsburgh, PA, USA) in RPMI-1640 media containing 7% fetal bovine serum (Life Technologies, Grand Island, NY, USA), 100 U/ml penicillin, 100 μg/ml streptomycin, 2 mM L-glutamine, 50 mM 2-ME, 1 mM sodium pyruvate, 0.01 mM nonessential amino acids, and 10 mM HEPES.
Univariate analysis was performed with Chi-Square testing for categorical variables (Fisher exact test used for violations of Cochran's assumptions) and student's t-test for continuous variables (Mann Whitney test was used for variables without a Gaussian distribution).
The results were compared between groups with ANOVAs followed by Fisher PLSD post-hoc tests.
Fisher's exact test was used to compare adverse event rate and ANOVA was used to compare change from baseline for laboratory variables between the two treatment arms.