Example sentences for: firefly

How can you use “firefly” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • In the rest of The Bounty , Walcott relinquishes such allusive density for the simple, history-erasing realities of life in the Antilles islands: the mango trees that "serenely rust when they are in flower," the firefly that "keeps striking matches."

  • To determine the luciferase activity, cells or tissues were lysed in Passive Lysis Buffer (Promega) and assayed for firefly (F-luc) and Renilla (R-Luc) luciferase according to the Promega Luciferase assay kit instructions in a Turner Designs (Sunnyvale, CA) TD-20/20 or a PharMingen Monolight 3010 luminometer.

  • A plasmid encoding the HBSAg under the control of the Human Cytomegalovirus Immediate Early gene I (HCMVIE1) enhancer/promoter (pRc/CMV-HBs(S) was obtained from Aldevron (Fargo, ND) [ 20 ] . A plasmid encoding the firefly luciferase gene under the control of the HCMVIE1 enhancer/promoter (pHCMVIE1-luciferase) was used to measure relative gene expression levels following gene transfer by low-volume jet injection, particle bombardment and needle and syringe injection [ 2 ] . Plasmids were propagated in DH5α cells and purified by Bio 101 Merlin Core Services (Carlsbad, CA).

  • To evaluate the reporter induction, the ratio of RLU from firefly (F-luc) and Renilla (R-Luc) luciferase was evaluated (RLU{F-luc}/RLU{R-luc}).

  • Analysis of genetic and molecular mechanisms underlying circadian clocks in whole animals or isolated tissues is facilitated by the use of molecular reporters, such as firefly luciferase ( luc ). The use of firefly- luciferase ( luc ) as a real-time molecular reporter of gene expression has been illustrated in a variety of model systems including cyanobacteria, plants, Drosophila , and mammals [ 15 16 17 18 ] . This method has proved especially valuable in studies about molecular rhythms because real-time expression of clock genes and other chronobiological factors may be monitored over many daily cycles in vivo [ 17 6 19 20 21 ] . Applications of this technique are varied.


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