Words similar to firefly
Example sentences for: firefly
How can you use “firefly” in a sentence? Here are some example sentences to help you improve your vocabulary:
A plasmid encoding the HBSAg under the control of the Human Cytomegalovirus Immediate Early gene I (HCMVIE1) enhancer/promoter (pRc/CMV-HBs(S) was obtained from Aldevron (Fargo, ND) [ 20 ] . A plasmid encoding the firefly luciferase gene under the control of the HCMVIE1 enhancer/promoter (pHCMVIE1-luciferase) was used to measure relative gene expression levels following gene transfer by low-volume jet injection, particle bombardment and needle and syringe injection [ 2 ] . Plasmids were propagated in DH5α cells and purified by Bio 101 Merlin Core Services (Carlsbad, CA).
To determine the luciferase activity, cells or tissues were lysed in Passive Lysis Buffer (Promega) and assayed for firefly (F-luc) and Renilla (R-Luc) luciferase according to the Promega Luciferase assay kit instructions in a Turner Designs (Sunnyvale, CA) TD-20/20 or a PharMingen Monolight 3010 luminometer.
[ 12 13 ] These bladder cell lines were transfected with a novel ecdysone system regulating the expression of firefly luciferase and then injected subcutaneously into syngeneic hosts.
Analysis of genetic and molecular mechanisms underlying circadian clocks in whole animals or isolated tissues is facilitated by the use of molecular reporters, such as firefly luciferase ( luc ). The use of firefly- luciferase ( luc ) as a real-time molecular reporter of gene expression has been illustrated in a variety of model systems including cyanobacteria, plants, Drosophila , and mammals [ 15 16 17 18 ] . This method has proved especially valuable in studies about molecular rhythms because real-time expression of clock genes and other chronobiological factors may be monitored over many daily cycles in vivo [ 17 6 19 20 21 ] . Applications of this technique are varied.
Cells were treated for 24 hours, at which time they were collected in Passive Lysis Buffer and the activity of firefly and Renilla luciferase in the cell extract was determined.
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