Words similar to firefly
Example sentences for: firefly
How can you use “firefly” in a sentence? Here are some example sentences to help you improve your vocabulary:
A plasmid encoding the HBSAg under the control of the Human Cytomegalovirus Immediate Early gene I (HCMVIE1) enhancer/promoter (pRc/CMV-HBs(S) was obtained from Aldevron (Fargo, ND) [ 20 ] . A plasmid encoding the firefly luciferase gene under the control of the HCMVIE1 enhancer/promoter (pHCMVIE1-luciferase) was used to measure relative gene expression levels following gene transfer by low-volume jet injection, particle bombardment and needle and syringe injection [ 2 ] . Plasmids were propagated in DH5α cells and purified by Bio 101 Merlin Core Services (Carlsbad, CA).
To evaluate the reporter induction, the ratio of RLU from firefly (F-luc) and Renilla (R-Luc) luciferase was evaluated (RLU{F-luc}/RLU{R-luc}).
To determine the luciferase activity, cells or tissues were lysed in Passive Lysis Buffer (Promega) and assayed for firefly (F-luc) and Renilla (R-Luc) luciferase according to the Promega Luciferase assay kit instructions in a Turner Designs (Sunnyvale, CA) TD-20/20 or a PharMingen Monolight 3010 luminometer.
[ 12 13 ] These bladder cell lines were transfected with a novel ecdysone system regulating the expression of firefly luciferase and then injected subcutaneously into syngeneic hosts.
Pathway activity was then assessed by measurement of luciferase expressed from a co-transfected plasmid containing firefly luciferase-coding sequence under the control of LEF binding elements.
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