Words similar to firefly
Example sentences for: firefly
How can you use “firefly” in a sentence? Here are some example sentences to help you improve your vocabulary:
Pathway activity was then assessed by measurement of luciferase expressed from a co-transfected plasmid containing firefly luciferase-coding sequence under the control of LEF binding elements.
[ 12 13 ] These bladder cell lines were transfected with a novel ecdysone system regulating the expression of firefly luciferase and then injected subcutaneously into syngeneic hosts.
To determine the luciferase activity, cells or tissues were lysed in Passive Lysis Buffer (Promega) and assayed for firefly (F-luc) and Renilla (R-Luc) luciferase according to the Promega Luciferase assay kit instructions in a Turner Designs (Sunnyvale, CA) TD-20/20 or a PharMingen Monolight 3010 luminometer.
These cells where in turn co-transfected with pSV40/Zeo2 (Invitrogen) and pG5-luc (Promega), encoding firefly luciferase or pGAL4-SEAP and MBT-2 cells were selected in 400 μg/ml Zeocin and Panc02 cells in 500 μg/ml Zeocin.
Analysis of genetic and molecular mechanisms underlying circadian clocks in whole animals or isolated tissues is facilitated by the use of molecular reporters, such as firefly luciferase ( luc ). The use of firefly- luciferase ( luc ) as a real-time molecular reporter of gene expression has been illustrated in a variety of model systems including cyanobacteria, plants, Drosophila , and mammals [ 15 16 17 18 ] . This method has proved especially valuable in studies about molecular rhythms because real-time expression of clock genes and other chronobiological factors may be monitored over many daily cycles in vivo [ 17 6 19 20 21 ] . Applications of this technique are varied.
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