Words similar to final
Example sentences for: final
How can you use “final” in a sentence? Here are some example sentences to help you improve your vocabulary:
The HCMV DNA was detected using HCMV1 (5' cct agt gtg gat gac cta cgg gcc a) and HCMV2 (5' cag aca cag tgt cct ccc gct cct c) primers producing 249 bp long amplicon and the DNA of HHV6 was amplified with specific primer pair HP0 (5' ccg caa tcg aat cca cct agc gg) and HP4 (5' gtg aga acg gat tcg aac agt gct g) yielding 440 bp product [ 23 24 ] . All amplifications were carried out with 20 pmol of each primer in 2 mM solution of MgCl 2 , 2 U of Tag Special DNA polymerase (Biovendor, Czech Republic), 0.3 mM of each dNTPs, 10× reaction buffer and 1 μg of isolated DNA according to the following conditions: 96°C for 4 min, (94°C for 10 sec, 58°C for 10 sec, 72°C for 20 sec) 36 times, and final extension at 72°C for 2 min.
Each amplification reaction contained 50 mM KCl, 10 mM tris-HCl pH 8.4, 1.5 mM MgCl 2 , 0.25 μM of each primer, 200 μM dNTP, 10% DMSO, 0.1 μg DNA and 1 U Tag polymerase in a final volume of 20 μl.
Conditions for exception would be: (l) better access to a sampling point between the final treatment and the discharge outfall; (2) if the processed waste is chlorinated prior to discharge, it may also be desirable to take samples prior to contact with the chlorine to determine toxicity of the unchlorinated effluent; or (3) in the event there is a desire to evaluate the toxicity of the influent to municipal waste treatment plants or separate wastewater streams in industrial facilities prior to their being combined with other wastewater streams or non-contact cooling water, additional sampling points may be chosen.
A copy of the final document, along with the carefully crafted instructions that were to be sent to the tribals, was given to Tenet.
On review, intra-observer disagreements appeared to be more due to the subjective choice of threshold for final segmentation rather than to disparities in the final background-leveled image to which the threshold was applied.
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