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Example sentences for: filament
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Myosin II heavy chain kinase (MHCK) activity in this system capable of disassembling myosin II filaments in vitro was originally reported with partially enriched kinase fractions [ 13 ] . The enzyme MHCK-A was subsequently purified to homogeneity and shown to be capable of driving myosin II filament disassembly in vitro via myosin II heavy chain phosphorylation [ 14 15 ] ). A MHCK-A cDNA was cloned via expression cloning and peptide sequence derived from the native enzyme [ 16 ] . This enzyme is now recognized as the founding member of a highly novel family of protein kinases unrelated to conventional protein kinases, with members present in D. discoideum and throughout the animal kingdom.
In the lens, alpha-crystallin also forms a complex with type III intermediate filament proteins and the lens-specific beaded filament proteins CP49 and CP115, which may be critical for proper filament assembly [ 16 ] . Beaded filament mRNA levels increase greatly in differentiating lens epithelial cells, and have been suggested as a pan-specific marker for lens fiber cells [ 17 ] . It is therefore possible that increased synthesis of alpha-crystallin in epithelial cells early in the differentiation process may have profound effects upon the cytoskeleton, which in turn may profoundly affect cell shape and migration.
Each filament was applied perpendicularly to the plantar surface of the ligated paw of rats kept in suspended wire-mesh cages.
Intermediate filament extracts of the cell lines [ 43 44 ] were electrophoretically blotted and detected with polyclonal anti GFAP (DAKO, Carpenteria, CA) and secondary reagents as above.
Samples were then subjected to centrifugation to sediment myosin II filaments, and the proportion of myosin II in the pellet in each sample was quantified using SDS-PAGE and Coomassie blue staining as a measure of filament assembly (Figure 3A).
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