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Example sentences for: filament
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Myosin II heavy chain kinase (MHCK) activity in this system capable of disassembling myosin II filaments in vitro was originally reported with partially enriched kinase fractions [ 13 ] . The enzyme MHCK-A was subsequently purified to homogeneity and shown to be capable of driving myosin II filament disassembly in vitro via myosin II heavy chain phosphorylation [ 14 15 ] ). A MHCK-A cDNA was cloned via expression cloning and peptide sequence derived from the native enzyme [ 16 ] . This enzyme is now recognized as the founding member of a highly novel family of protein kinases unrelated to conventional protein kinases, with members present in D. discoideum and throughout the animal kingdom.
The question of how myosin II thick filament assembly is regulated within living cells, however, remains mostly unanswered.
We suggest that differential localization of MHCKs occurs in D. discoideum cells for the purpose of regulating myosin II filament assembly levels in the context of specific cellular contractile events such as lamellipodium extension and cytokinetic furrowing.
This result also suggested that Triton X-100 extraction of cells may not preserve moesin-actin filament complexes.
The pTX-MKC2 plasmid was therefore transfected into 3xALA myosin II cells, which are resistant to myosin filament hyperphosphorylation and disassembly due to elimination of phosphorylation target sites in the myosin tail [ 24 ] . The resultant 3xALA/pTX-MKC2 cells could be propagated in suspension culture even after selection for elevated expression in 40 μg/ml G418.
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