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Example sentences for: ferric
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Examples of donor-acceptor combinations might include: simple sugars and/or amino acids or other organic molecules in the presence of oxygen or nitrate (organisms capable of heterotrophic respiration), hydrogen and ferric ions or oxidized iron minerals (chemoautotrophic hydrogen oxidizers), organic acids and ferric ions or oxidized iron minerals (heterotrophic iron reducers), bicarbonate/carbonate and trace minerals in the presence and absence of light (photosynthetic organisms), carbon dioxide and a reduced metal such as ferrous ions (chemoautotrophs), ammonium ion and bicarbonate/carbonate (ammonia oxidizers), etc.
M. paratuberculosis ATCC19698 was grown in Middlebrook 7H9 broth (pH 6.0) supplemented with oleic acid albumin dextrose complex (Becton Dickinson Microbiology) and 0.05% Tween 80 and ferric mycobactin J (2 mg/L).
Pyridine-2,6-bis(thiocarboxylic acid) (pdtc) is an iron-regulated metabolite produced by certain pseudomonads that is highly reactive towards metals by virtue of a pair of thiocarboxylate groups and ring nitrogen that combine to form a non-specific, high affinity, tri-dentate ligand (Figure 1) [ 1 2 ] . The formation constants for Co, Cu, and Fe complexes of pdtc were determined to be greater than 10 33 [ 3 ] . Uptake studies using 59Fe showed that addition of pdtc to the growth medium of two different pdtc-producing pseudomonads increased the efficiency of iron uptake by 20% [ 1 ] . The contribution of pdtc to iron uptake may be due to the fact that the copper complex of pdtc (Cu:pdtc) has the ability to reduce both soluble and mineral forms of ferric iron) [ 1 ] . Not surprisingly, in light of its high affinity for essential metals, pdtc also exhibits antimicrobial activity [ 4 ] .
[ 40 ] . Its purity and state of oxidation was confirmed by an oxidimetric titration using arsenite-cerate method [ 41 ] . This method measures only Fe(VI and V), leaving any ferric ions in the preparation unreduced.
For analysis of Biolog™ PM3 plates 150 μl ferric citrate was also added to each tube of inoculating fluid.