Example sentences for: fcs

How can you use “fcs” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The bacteria from each culture were harvested by centrifugation for 1 minute at 16,000 × g, washed twice with RPMI 1640/10% FCS, and resuspended in RPMI 1640/10% FCS at a concentration of 1.5 × 10 8bacteria/mL.

  • These cells were cultured in RPMI medium supplemented with 10% FCS, 2 mM L-glutamine, and 0.05 mM 2-mercaptoethanol without antibiotic/antimycotic.

  • Human Embryonic Kidney (HEK) 293 cells were maintained in D-MEM/F-12 (Dulbecco's Modified Eagle's Medium: Nutrient Mixture F-12 1:1 mixture), supplemented with 10% (v/v) fetal calf serum (FCS), 200 μg/ml G-418 (Geneticin ®) in a humidified incubator with 5% CO 2 and 95% air, at 37°C.

  • To kill any remaining extracellular bacteria, 3 mL of fresh RPMI 1640/10% FCS/200 μg amikacin/mL (Sigma) was added to each well.

  • Dexamethasone, ascorbic acid-2 phosphate, β-glycerophosphate, bovine serum albumin (BSA), and FCS were purchased from Sigma Diagnostics (St Louis, MO, USA); penicillin, streptomycin, DMEM, and RPMI-1640 from Biowhittaker (Watersville, MD, USA); Lymphoprep ™from Nycomed, Oslo, Norway.


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