Words similar to fat-free

Example sentences for: fat-free

How can you use “fat-free” in a sentence? Here are some example sentences to help you improve your vocabulary:

The blots were blocked in 5% fat-free milk in PBS-Tween at room temperature for 1 hour, then exposed to primary antibodies (mouse anti-human MMP-1, MMP-2, MMP-3 or MMP-9 antibodies; Calbiochem, Cambridge, MA, USA), and subsequently detected using HRP conjugated rabbit anti-mouse IgG (ICN Biomedical, Costa Mesa, CA, USA) in conjunction with an enhanced chemiluminescence detection system (ECL, Amersham Pharmacia Biotech, Little Chalfont, Buckinghamshire, England).
05% Tween 20 and biotin labeled anti-mouse IgG (100 μl/well; 125 ng/ml diluted in PBS containing 1% dry fat-free milk) was added.
Blots were blocked at room temperature for 1 hour in TBS-T (10 mM Tris, pH 8.0, 150 mM NaCl, 0.1% Tween-20) containing 5% dried fat-free milk, then incubated overnight at 4°C in TBS-T containing 0.5% milk and primary Ab (0.
1 μg protein/100 μl per well) for ELISA, and free binding capacity of the wells was blocked by 1% fat-free milk in phosphate-buffered saline [ 23 24 25 ] . Sera were applied at increasing dilutions and isotypes of proteoglycan-specific antibodies were determined using peroxidase-conjugated rat antimouse IgG 1 or IgG 2a secondary antibodies (Zymed, South San Francisco, CA, USA) as previously described [ 24 26 ] . Serum antibody levels were calculated according to mouse IgG 1 and IgG 2a standards.
SCD, sickle cell disease; R, resistance, Xc, reactance; MMP, mean melting point; FFM, fat-free mass; BMI, body mass index; DHA, docosahexaenoic acid; PC, phosphatidylcholine.