Words similar to falciparum
Example sentences for: falciparum
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P. falciparum (3D7 or Dd2) was grown in human A-positive erythrocytes and infected cells were harvested at about 15% parasitemia and lysed by the saponin method essentially as described [ 14 15 ] . PfPP5 was partially purified using published procedures [ 18 ] . In brief, the parasites were resuspended in buffer A (50 mM Tris-Cl, pH 7.5, 10% glycerol, 1 mM DTT) plus 20 mM NaCl, 1% Triton X-100, leupeptin (1 μg/ml), and aprotinin (1 μg/ml) at an approximate protein concentration of 10 mg per ml.
81 ± 3.58/(μl) were found positive for P. falciparum by rapid test.
Rapid and accurate methods are needed for the diagnosis of Plasmodium falciparum malaria [ 1 2 ] as malaria is estimated to kill between 1.4 and 2.6 million people each year [ 1 ] . WHO South East Asian regional office estimates 19500 deaths due to malaria in India annually [ 3 ] . P. falciparum infections in children may become life threatening making rapid diagnosis of extreme importance [ 4 ] . No clinical diagnostic criteria are both sensitive and specific for malaria, an undifferentiated febrile illness [ 5 ] which may result in the erroneous treatment of millions of non-malaria cases with antimalarial drugs in the absence of diagnostic malaria microscopy.
Several well-characterized plasmodial genes were identified in this metabolic collection, including P. falciparum lactate dehydrogenase (PfLDH; 027743), enolase (U00152), triose-phosphate isomerase (PfTPI; L01654), glucose-6-phosphate isomerase (PfG6PI; J05544), hypoxanthine-guanine phosphoribosyl-transferase (PfHGPRT; X16279) and dihydropteroate synthetase (PfDHPS; U07706).
Two additional gene groups whose functions are linked to the process of protein synthesis were present among the trophozoite genes: five DEAD-box RNA helicases, including a close homolog of P. cynomolgi RNA helicases-1 [ 41] and 23 molecular chaperone-like molecules, including two P. falciparum heat-shock proteins such as PfHSP70 (GenBank accession number M19753) and PfHSP86 (accession number L34028), and a homolog of a DnaJ-domain-containing protein family, DNJ1/SIS1 homolog [ 42].
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