Words similar to eyfp

Example sentences for: eyfp

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For whole mount photographs (Figures 2and 4), unfixed embryos were photographed using a Nikon epifluorescence microscope fitted with Chroma filter sets for ECFP (cyan GFP Ex436/20 Dm455 Bar480/40) and EYFP (yellow GFP Ex500/20 Dm515 Bar535/30).
However, when the reporter mice were crossed to β-actin-Cre transgenic mice [ 23], which express Cre constitutively in most or all cells including those in the early embryo, the resulting doubly transgenic embryos displayed apparently ubiquitous expression of EYFP or ECFP (Figure 2), indicating efficient excision of the floxed stop sequence.
In contrast to lacZ expression, which cannot be easily detected in living tissue, the EYFP and ECFP reporter strains (together with the GFP reporter strains currently available) will be very useful for monitoring the expression of Cre in living tissues, or tracing the lineage of these cells and their descendants, in cultured embryos or organs.
The plasmids pEYFP-N1 and pECFP containing cDNA for EYFP and ECFP were purchased from Clontech Laboratories Inc.
ECFP/EYFP fluorescence changes with respect to time for regions of interest corresponding to an area of Golgi membrane were determined by calculating the F t /F 0 , where F t is the background- and bleach-corrected ECFP or EYFP fluorescence at time = t and F 0 is the background-corrected ECFP or EYFP fluorescence at time = 0 s.