Words similar to eyfp
Example sentences for: eyfp
How can you use “eyfp” in a sentence? Here are some example sentences to help you improve your vocabulary:
To make the R26R-YFP targeting construct, the EYFP cDNA was excised from pEYFP-N1 with ApaI and NotI and inserted into BigT digested with ApaI and NotI.
We have constructed two reporter lines of mice that express EYFP or ECFP only in cells expressing the Cre recombinase, and their daughter cells, by targeting these cDNAs into the ubiquitously expressed ROSA26 locus, preceded by a loxP flanked "stop" sequence.
BigT was then digested with PacI and AscI to release the entire floxed neo -tpA and EYFP assembly, and inserted into pROSA26PA digested with PacI and AscI.
ECFP/EYFP fluorescence changes with respect to time for regions of interest corresponding to an area of Golgi membrane were determined by calculating the F t /F 0 , where F t is the background- and bleach-corrected ECFP or EYFP fluorescence at time = t and F 0 is the background-corrected ECFP or EYFP fluorescence at time = 0 s.
Different fluorescent-protein tagged constructs were produced by exchanging the NheI/BsrGI fragment containing the fluorescent protein coding sequence between EGFP, EYFP, and ECFP.