Example sentences for: eyfp

How can you use “eyfp” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • We have constructed two reporter lines of mice that express EYFP or ECFP only in cells expressing the Cre recombinase, and their daughter cells, by targeting these cDNAs into the ubiquitously expressed ROSA26 locus, preceded by a loxP flanked "stop" sequence.

  • ECFP or EYFP was inserted into pBigT, and the resulting sequence was inserted into a vector containing ROSA26 genomic sequences to permit homologous recombination (Fig 1A).

  • Different fluorescent-protein tagged constructs were produced by exchanging the NheI/BsrGI fragment containing the fluorescent protein coding sequence between EGFP, EYFP, and ECFP.

  • EYFP and ECFP were chosen because their emission spectra overlap minimally, so they can be distinguished when used simultaneously, whereas the emission spectra of EYFP and ECFP overlap to a greater extent with that of EGFP [ 16, 17] http://www.clontech.com/gfp/pdf/LivingColors.pdf.

  • This ubiquitous expression of EYFP or ECFP was heritably transmitted to the following generations (data not shown), resulting in ubiquitously expressing lines of mice that will be useful for lineage tracing in chimeric mice or embryos, analogous to the original ROSA26 lacZ promoter trap strain [ 21].


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