Example sentences for: eyfp

How can you use “eyfp” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • To investigate this possibility, cells were transfected with a wild-type cPLA 2 fused to EYFP (EYFP-cPLA 2 ) and the distribution of EYFP-cPLA 2 was imaged in response to [Ca 2+] i transients elicited by ATP and sustained [Ca 2+] i elevations elicited by IONO, in the presence and absence of U0126.

  • We have constructed two reporter lines of mice that express EYFP or ECFP only in cells expressing the Cre recombinase, and their daughter cells, by targeting these cDNAs into the ubiquitously expressed ROSA26 locus, preceded by a loxP flanked "stop" sequence.

  • To make the R26R-YFP targeting construct, the EYFP cDNA was excised from pEYFP-N1 with ApaI and NotI and inserted into BigT digested with ApaI and NotI.

  • Different fluorescent-protein tagged constructs were produced by exchanging the NheI/BsrGI fragment containing the fluorescent protein coding sequence between EGFP, EYFP, and ECFP.

  • ECFP or EYFP was inserted into pBigT, and the resulting sequence was inserted into a vector containing ROSA26 genomic sequences to permit homologous recombination (Fig 1A).


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