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Example sentences for: expresshyb

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The second was the random primer-labeled LacI/NLS . Membranes were prehybridized for 0.5 h at 65°C, hybridized for 1 h at 65°C in ExpressHyb hybridization solution and washed at 60°C in washing solution.
Blots were prehybridized and hybridized at 42°C in ExpressHyb (Clontech, Palo Alto, CA) and washed in 0.1× SSC/1% SDS for one hour at 65°C.
Hybridization was done in ExpressHyb solution (Clontech) at 60°C using a 32P dCTP-labeled probe that was synthesized from a 0.9 Kb template located in intron 5 of Pten [ 25] using the RTS RadPrime DNA labeling system (Life Technologies).
Membranes were hybridized with labeled-probes (see above) in ExpressHyb (Clontech) overnight at 65°C, washed and exposed to X-ray film with an intensifying screen at -80°C.
To hybridize the arrays, purified and labeled cDNA targets were dissolved in 100 μl total volume of ExpressHyb solution (Clontech, Palo Alto, CA) containing 8 μg of polydA 40-60 (Amersham Pharmacia), 2 μg of yeast tRNA (Gibco-BRL), 10 μg of human Cot I DNA (Gibco-BRL).