Words similar to expresshyb

Example sentences for: expresshyb

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Hybridization was done in ExpressHyb solution (Clontech) at 60°C using a 32P dCTP-labeled probe that was synthesized from a 0.9 Kb template located in intron 5 of Pten [ 25] using the RTS RadPrime DNA labeling system (Life Technologies).
To hybridize the arrays, purified and labeled cDNA targets were dissolved in 100 μl total volume of ExpressHyb solution (Clontech, Palo Alto, CA) containing 8 μg of polydA 40-60 (Amersham Pharmacia), 2 μg of yeast tRNA (Gibco-BRL), 10 μg of human Cot I DNA (Gibco-BRL).
The membrane was prehybridized for 0.5 h at 65°C, hybridized for 1 h at 65°C with random primer-labeled lac operator concatamer (40 copies) probe in ExpressHyb hybridization solution (Clontech Laboratories, CA), and then washed at 60°C in washing solution (0.
Samples were hybridized in manufacturer-supplied hybridization solution (Clontech ExpressHyb) for 30 min at 68°C.
Blots were prehybridized and hybridized at 42°C in ExpressHyb (Clontech, Palo Alto, CA) and washed in 0.1× SSC/1% SDS for one hour at 65°C.