Words similar to expresshyb
Example sentences for: expresshyb
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Membranes were hybridized with labeled-probes (see above) in ExpressHyb (Clontech) overnight at 65°C, washed and exposed to X-ray film with an intensifying screen at -80°C.
Blots were prehybridized and hybridized at 42°C in ExpressHyb (Clontech, Palo Alto, CA) and washed in 0.1× SSC/1% SDS for one hour at 65°C.
Hybridization was done in ExpressHyb solution (Clontech) at 60°C using a 32P dCTP-labeled probe that was synthesized from a 0.9 Kb template located in intron 5 of Pten [ 25] using the RTS RadPrime DNA labeling system (Life Technologies).
To hybridize the arrays, purified and labeled cDNA targets were dissolved in 100 μl total volume of ExpressHyb solution (Clontech, Palo Alto, CA) containing 8 μg of polydA 40-60 (Amersham Pharmacia), 2 μg of yeast tRNA (Gibco-BRL), 10 μg of human Cot I DNA (Gibco-BRL).
Samples were hybridized in manufacturer-supplied hybridization solution (Clontech ExpressHyb) for 30 min at 68°C.