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Example sentences for: expressers
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When all of the approximately 9,000 probes are divided into three equally sized bins representing low, medium, and high expressers according to their respective signal intensities, the effects of increased hybridization time and DAP become very evident (Figure 3C).
A lower limit of detection was previously defined using our platform by developing a negative control threshold in order to assign a confidence level to such signal or noise queries [ 6 ] . Briefly, this threshold was determined by taking the mean signal of bacterial negative control probes mentioned above (minus a 10% trim to account for weak cross hybridization of high expressers or for true hybridization to sequences not in the database) and adding three standard deviations (99.
In summary, incorporation of modified nucleotides may not only lead to discovery and better quantitation of rare expressers in complex samples but also impact other aspects of the microarray experiment such as probe design prior to array fabrication and the statistical design of microarray experiments.
As shown in Figures 2Band 2C, the largest increases in signal intensities occurred for low and medium expressers relative to the unmodified control sample.
Figure 3Cshows that the relative increases in signal intensities due to increased hybridization time are biased towards the medium and high expressers, with signals increasing an average of 48% and 84%, respectively, whereas increases due to DAP substitution are more pronounced for low and medium expressers (57% and 81% increase, respectively).
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