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Example sentences for: explants

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Intermediate regions of the open neural tube (i-explants) were dissected from stage 10-11 chick embryos and embedded in collagen gel [ 23 ] . Explants were cultured in Ham-F12 supplemented with 3 g/l D-glucose, Mito Serum Extender (Collaborative Research; Bedford, USA), penicillin/streptomycin (Gibco), 2 mM L-glutamine (Gibco) and Hh-Ag 1.3 (0.
9B), BMP1, BMP3b, BMP8a, Smad7, and GR best discriminate control from SN50-treated explants.
12B), PNN analysis demonstrates that increased expression of FAF and Caspase 6 best discriminates control from SN50-treated explants.
To confirm these results, we repeated key experiments with insulin and the signal transduction inhibitors using human fetal lung explants.
Our group previously observed spatially uniform recovery of explants following trypsin-induced GAG depletion using dGEMRIC, with the increases in [GAG] occurring most rapidly during the first week and slowing considerably after 3 weeks [ 14 ] . Williams et al . [ 15 ] used the same method to monitor GAG accumulation in tissue engineered cartilage over 6 weeks, and observed relatively steady GAG accumulation over the entire period, with the bulk of the accumulation occurring at the periphery of the explant.