Words similar to ethanesulfonic
Example sentences for: ethanesulfonic
How can you use “ethanesulfonic” in a sentence? Here are some example sentences to help you improve your vocabulary:
They were routinely grown in a monolayer (30%-50% confluence) on a glass cover slide at 37°C in DMEM (Dulbecco's Modified Eagle Medium), supplemented with 1% L -glutamine, 1% HEPES [4-(2-hydroxyethyl)piperazine-1-(2-ethanesulfonic acid)], 0.5% gentamicin, 1% penicillin/streptomycin and 10% Fetal Bovine Serum (Gibco, NY).
pR, proteorhodopsin; bR, bacteriorhodopsin; OG, β-octyl-D-glucoside; SDS, sodium dodecylsulfate; PAGE, polyacrylamide gel electrophoresis; pR-TCM, pR triple cysteine mutant [C(107,156,175)S]; DHPC, 1,2-diheptanoyl-SN-glycero-3-phosphocholine; DMPC, dimyristoylphosphatidylcholine; HEPES, N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid
The supernatant was assayed for caspase activity in 20 mM Piperazine-N,N'-bis(2-ethanesulfonic acid) (pH 6.8), 100 mM NaCl, 1 mM EDTA, 1% CHAPS, and 10% sucrose with Z-IETD-AFC for caspase 8 [ 42 43 ] . The release of the fluorogenic-leaving group was detected by excitation at 400 nm and emission at 505 nm.
At the time of experimental treatment, the culture media was replaced with Locke's buffer containing (in mM) 154 NaCl, 5.6 KCl, 2.3 CaCl 2 , 1 MgCl 2 , 3.6 NaHCO 3 , 5 glucose, 5 N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (pH 7.2) and neurons were incubated with culture supernatants from HIV infected monocytic cells, subtoxic concentrations of Tat (40 nM) + gp120 (30 pM), various concentrations of methamphetamine or cocaine, or viral proteins + methamphetamine and cocaine.
BCIP = 5-bromo-4-chloroindol-3-yl phosphate; cDNA = complementary DNA; EGTA = (ethylene-bis [oxyethylenenitrilo])tetraacetic acid; CHTN = Cooperative Human Tissue Network; ELISA = enzyme-linked immunosorbent assay; MAP = microtubule associated protein; MES = 2-( N -morpholino)-ethane sulfonic acid; NBT = nitro blue tetrazolium; PBS = phosphate-buffered saline; PC-tubulin = MAP-free phosphocellulose-purified tubulin; PCR = polymerase chain reaction; PIPES = piperazine- N,N '-bis(2-ethanesulfonic acid); PVDF = poly(vinylidene difluoride).
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