Example sentences for: ethanesulfonic

How can you use “ethanesulfonic” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Prehybridization was performed in a solution containing 60% formamide, 0.1 M piperazine- N,N' -bis(2-ethanesulfonic acid) pH 6.4, 0.4 M NaCl, and 0.2 mg/ml of E. coli tRNA (Sigma, St. Louis, MO) for 2 h at 55°C with gentle shaking.

  • They were routinely grown in a monolayer (30%-50% confluence) on a glass cover slide at 37°C in DMEM (Dulbecco's Modified Eagle Medium), supplemented with 1% L -glutamine, 1% HEPES [4-(2-hydroxyethyl)piperazine-1-(2-ethanesulfonic acid)], 0.5% gentamicin, 1% penicillin/streptomycin and 10% Fetal Bovine Serum (Gibco, NY).

  • The supernatant was assayed for caspase activity in 20 mM Piperazine-N,N'-bis(2-ethanesulfonic acid) (pH 6.8), 100 mM NaCl, 1 mM EDTA, 1% CHAPS, and 10% sucrose with Z-IETD-AFC for caspase 8 [ 42 43 ] . The release of the fluorogenic-leaving group was detected by excitation at 400 nm and emission at 505 nm.

  • MgSO 4 , (ethylene-bis [oxyethylenenitrilo])tetraacetic acid (EGTA), GTP (type II-S), glycerol, 2-( N -morpholino)-ethane sulfonic acid (MES), Nonidet P 40, piperazine- N,N '-bis(2-ethanesulfonic acid) (PIPES) and Triton X-100 were purchased from Sigma Chemical Company.

  • pR, proteorhodopsin; bR, bacteriorhodopsin; OG, β-octyl-D-glucoside; SDS, sodium dodecylsulfate; PAGE, polyacrylamide gel electrophoresis; pR-TCM, pR triple cysteine mutant [C(107,156,175)S]; DHPC, 1,2-diheptanoyl-SN-glycero-3-phosphocholine; DMPC, dimyristoylphosphatidylcholine; HEPES, N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid


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