Example sentences for: ethanesulfonic

How can you use “ethanesulfonic” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • At the time of experimental treatment, the culture media was replaced with Locke's buffer containing (in mM) 154 NaCl, 5.6 KCl, 2.3 CaCl 2 , 1 MgCl 2 , 3.6 NaHCO 3 , 5 glucose, 5 N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (pH 7.2) and neurons were incubated with culture supernatants from HIV infected monocytic cells, subtoxic concentrations of Tat (40 nM) + gp120 (30 pM), various concentrations of methamphetamine or cocaine, or viral proteins + methamphetamine and cocaine.

  • pR, proteorhodopsin; bR, bacteriorhodopsin; OG, β-octyl-D-glucoside; SDS, sodium dodecylsulfate; PAGE, polyacrylamide gel electrophoresis; pR-TCM, pR triple cysteine mutant [C(107,156,175)S]; DHPC, 1,2-diheptanoyl-SN-glycero-3-phosphocholine; DMPC, dimyristoylphosphatidylcholine; HEPES, N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid

  • Cells were washed twice with a 1:1 mixture of Ham F10 nutrient medium and Dulbecco modified Eagle medium (DMEM) containing 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES, 25 mM), penicillin (50 U/ml) and streptomycin (50 μg/ml).

  • THP-1 (generously provided by Dr P Guyre, Dartmouth Medical School), Jurkat (ATCC), and 293 cell lines were cultured in RPMI complete medium (CM), consisting of RPMI 1640 with 10% heat-inactivated fetal bovine serum, 0.1 ml of sodium pyruvate, 2.0 mmol/l of L-glutamine, 25 mmol/l of 1 M HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid), 0.1 mmol/l of nonessential amino acids, 0.25 μg/ml of amphotericin, 50 μg/ml gentamicin (all from BioWhittaker, Walkersville, MD, USA) and 5 × 10 -5M 2-mercaptoethanol (Sigma, St Louis, MO, USA).

  • Prehybridization was performed in a solution containing 60% formamide, 0.1 M piperazine- N,N' -bis(2-ethanesulfonic acid) pH 6.4, 0.4 M NaCl, and 0.2 mg/ml of E. coli tRNA (Sigma, St. Louis, MO) for 2 h at 55°C with gentle shaking.


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