Example sentences for: ests

How can you use “ests” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Beginning with 5,713 traces, automated screens and manual inspection of misassembled contigs resulted in the elimination of 52 ESTs as potential chimeric sequences.

  • The outer (gray) panel displays computational evidence to support these annotations, such as gene predictions generated by programs such as GenScan [ 44] and Genie [ 45], and other biological data, such as ESTs aligned using the program Sim4 [ 2] and protein homologies revealed by BLASTX [ 1].

  • The generation of expressed sequence tags (ESTs) by single-pass random sequencing of cDNA libraries is a powerful tool for rapid gene transcript identification in metazoans [ 14 15 16 17 ] including parasitic nematodes of humans and animals [ 18 19 20 21 22 23 ] . High-throughput projects on two dozen nematode species have now brought the total number of publicly available roundworm ESTs to nearly 400,000, with half the sequences coming from parasites [ 24 25 26 27 ] . As a part of these efforts, EST sequencing from plant parasitic nematodes is in progress [ 28 ] and pilot EST datasets from the root-knot nematode M. incognita and the cyst nematodes Globodera rostochiensis and G. pallida second-stage larvae have recently been analyzed [ 29 30 ] .

  • This same clustering methodology is being applied to ESTs from other nematode species [ 32 ] .

  • To assess the quality of directional cloning in EST libraries, we applied the MEGABLAST tool [ 17] to align ESTs to the BOU of the UniGene cluster to which they belonged.


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