Words similar to equilibrated
Example sentences for: equilibrated
How can you use “equilibrated” in a sentence? Here are some example sentences to help you improve your vocabulary:
Qiagen Ni-NTA Superflow resin was equilibrated with modified buffer B before being combined with the cell lysate.
Pooled Macroprep Q fractions containing GFP-BLM were loaded onto a 5 ml HiTrap metal chelate column (Amersham Pharmacia) charged with NiS0 4 equilibrated in Buffer B. The metal chelate resin was washed with successive 10 column volumes of Buffer B + 10mm imidazole, Buffer B + 50 mM imidazole, and Buffer B + l00 mM imidazole.
Hammond Drierite Co, Ltd, Xenia, OH) to prevent water condensation, and the boxes with slides were equilibrated to room temperature before opening.
They were then applied on a heparin-Sepharose column (bed volume 5 ml) that was equilibrated and run (flow rate 20 ml/h) with a buffer consisted of 50 mM sodium acetate, pH 5.6, containing 0.25 M NaCl and 0.01% Tween-20.
Precipiates were washed four times in 20 mM Tris/HCl, pH 7.4; 150 mM NaCl; 0.5 % Triton X-100, 10 μg/ml leupeptin, 10 μg/ml pepstatin, 5 μg/ml aprotinin, 1 mM PMS, and equilibrated in 20 mM HEPES, pH 7.4, 100 mM potassium acetate, 1 mM DTT.