Words similar to equilibrated

Example sentences for: equilibrated

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The gel was then equilibrated in transfer buffer for 15 min and transferred to a polyvinylidine difluoride membrane (Novex, San Diego, CA), also equilibrated in transfer buffer for 15 min, for 1 hr at 100 V. The membrane was blocked overnight with 5% non-fat milk in PBS at 4°C, and incubated with a rabbit polyclonal antibody (1:2000) against a peptide (CPRPNREEPVDSRTP) at the C-terminal region of MECP2 (Upstate Biotechnology, Lake Placid, NY) at room temperature for 2 h in a western wash solution (PBS containing 2% non-fat milk, 0.1% Tween 20, and 350 mM NaCl).
Gels were either stained by Coomassie Brilliant Blue or equilibrated to be transferred into transfer buffer (0.
They were then equilibrated in a 1:1 mixture of OCT (Tissue-Tek, Mile, Inc.) and 15% sucrose in PBS for >1 hour, and embedded in OCT over dry ice.
Tissue sections were fixed for 30 min in 4% paraformaldehyde in DEPC-treated PBS, washed twice for 15 min in PBS containing 0.1% active DEPC, and equilibrated for 15 min in DEPC-treated 5x SSC.
Before hybridization, the cRNA samples were heated to 94°C for 5 min, equilibrated to 40°C for 5 min, and clarified by centrifugation at 14,000 g for 5 min.