Words similar to equilibrated

Example sentences for: equilibrated

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Slides were then washed twice for 15 min in TBS, and equilibrated for 5 min in TBS pH 9.5 (100 mM Tris, 100 mM NaCl, 50 mM MgCl 2 ). They were then stained in NBT/BCIP solution (Pierce, Cat No.
At the conclusion of each experimental series, two samples (one control and one treated) were equilibrated in gadolinium-free medium, then extracted from media and blotted dry, and placed in an NMR tube.
A 2.5 × 16 cm Bio-Gel HTP column previously equilibrated with 5 mM phosphate buffer (pH 7.0) containing 1 mM DTT was loaded with a 240-mg amount of protein, washed with 500 ml of starting buffer, and bound proteins were eluted with 800 ml of phosphate buffer at pH 7.0 (potassium phosphate gradient from 5-400 mM).
This solution was analyzed on an HPLC column equilibrated with 0.03 M TEAA (Solvent A) at a flow rate of 1 ml/min.
The gel was then equilibrated in transfer buffer for 15 min and transferred to a polyvinylidine difluoride membrane (Novex, San Diego, CA), also equilibrated in transfer buffer for 15 min, for 1 hr at 100 V. The membrane was blocked overnight with 5% non-fat milk in PBS at 4°C, and incubated with a rabbit polyclonal antibody (1:2000) against a peptide (CPRPNREEPVDSRTP) at the C-terminal region of MECP2 (Upstate Biotechnology, Lake Placid, NY) at room temperature for 2 h in a western wash solution (PBS containing 2% non-fat milk, 0.1% Tween 20, and 350 mM NaCl).