Words similar to eppendorf
Example sentences for: eppendorf
How can you use “eppendorf” in a sentence? Here are some example sentences to help you improve your vocabulary:
Sterile and pyrogen-free Eppendorf Biopur pipette tips were purchased from Brinkmann Instruments, Inc. (Westbury, NY).
At the time of analysis, 1.5 ml of the transfected cells were transferred to eppendorf tubes, centrifuged and lysed in 320 μl of lysis buffer (60 mM Na2HPO4, 40 mM NaH2PO4, 10 mM KCl, 1 mM MgSO4, and 0.1% Triton X-100 at pH7).
Electroporation of DNA into Y. pestis was done as described previously [ 32 ] . Gene amplification was performed with DeepVent (New England Biolabs, Beverly, MA) or Taq (Eppendorf Scientific, Westbury, NY) DNA polymerase in a Perkin-Elmer GeneAmp Model 2400 thermocycler (Applied Biosystems, Foster City, CA).
The resulting nuclear pellet was resuspended in 20 μl of Buffer C (20 mM K-HEPES pH 7.9, 1.5 mM MgCl 2 , 0.2 mM EDTA, 20% v/v glycerol, and 0.42 M NaCl, with protease inhibitors as above), incubated on ice for 20 minutes, then microcentrifuged (5 minutes at 14,000 rpm, Eppendorf 5415C, 4°C).
The cells were transferred to microfuge tubes, pelleted (5 seconds at 14,000 rpm, Eppendorf 5415C, 4°C) and resuspended in hypotonic Buffer A (10 mM K-HEPES pH 7.9, 1.5 mM MgCl 2 , and 10 mM KCl, with inhibitors added as for the media above) for 15 minutes on ice.
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