Example sentences for: epicentre

How can you use “epicentre” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 50 ng to 1 μg of either genomic DNA or WGA genomic sample was mixed with OCP (typically 0.5 nM final concentration) and 0.5 unit of Ampligase (Epicentre Technologies, Madison, WI) in 1x Ampligase buffer (Epicentre), for a total volume of 10 μl.

  • All ligation reactions were in 1x Ampligase Reaction Buffer supplied by Epicentre Technologies: 20 mM Tris-HCl (pH8.

  • RNA was isolated from an overnight culture using a MasterPure RNA purification kit (Epicentre, Madison, WI) and amplified by RT-PCR using primers KM37 and GM24 following the protocol provided for Tth polymerase.

  • SequiTherm EXCEL II DNA sequencing kit (Epicentre Technologies, Madison, WI) was used according to the manufacturer's instruction for the corresponding sequencing reaction using the same primer.

  • Biotinylated cRNA was prepared using the standard Affymetrix protocol [ 8 ] except that the Epicentre AmpliScribe™ T7 polymerase (Epicentre, Madison, WI) was substituted for the ENZO T7 polymerase (BioArray, High Yield RNA Transcript Labeling Kit, ENZO Diagnostics, Inc., Farmingdale, NY) for 2 of the treated and 2 of the untreated pooled samples.


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