Words similar to enucleation

Example sentences for: enucleation

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5 crx -/-and crx +/+littermate libraries were made directly from Trizol-purified total RNA and were the same starting material used in two previous studies from this lab [ 14 27 ] . Human tissue was obtained from patients with periocular malignancies, removal of which required enucleation of a healthy eye.
5°C until enucleation.
After enucleation, a donor cell was inserted into the perivitelline space of each enucleated oocyte using the same glass pipette.
Immediately after enucleation the eyes were fixed using 2.5% gludaraldehyde in 0.1 mol/l cacodylate buffer (pH 7.4).
The oocytes had been previously stained in the manipulation medium supplemented with 5 μg/ml Hoechst 33342 (Sigma) for 20 min and confirmation of successful enucleation was achieved by visualizing the cytoplast and removed cytoplasm under ultraviolet light.