Words similar to enterocyte
Example sentences for: enterocyte
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This regulatory genomic response of the enterocyte would result in an increase in the brush border membrane content of CaT1 calcium influx channels that could function as a gatekeeper mechanism to augment the rate of calcium entry across the apical membrane [ 9 ] . However, given that there is a Ca-dependent negative feedback mechanism to limit calcium entry through the channel by maintaining the channel in the closed position [ 14 15 ] there must be a way to remove calcium from the vicinity of the cytosolic face of the channel to optimize and sustain calcium influx rates.
Additionally, while screening an enterocyte cDNA library for full length cDNAs using PCR, we consistently amplified two DNA products when using Abcg8 primers flanking exon 2; one of the expected size, and another very low copy product ~60 bases larger.
The resulting enzyme is both mislocalized and unstable [ 27 ] . Another mutation, Q1098P, was found to cause retention of SI in the transport pathway between the endoplasmic reticulum and Golgi apparatus [ 28 ] . An unusual mutation, Q117R, that alters the structure close to the O-glycosylated stalk domain, leads to random distribution of the enzyme to both the apical and basolateral cell membranes [ 29 ] . An unusual human mutation that causes SI to be secreted rather than retained in the plasma membrane demonstrates that the enzyme is required to be at the enterocyte cell surface [ 30 ] .
Rat cDNAs for Abcg5 and Abcg8 were identified previously by amplification of these sequences from a Sprague Dawley rat enterocyte cDNA library, using primers located in areas conserved between mouse and human (Genbank AF312714 and AF351785 respectively).
Changes in the rate of vectorial Ca transport in the intestine however must be accomplished without a major disruption in the free intracellular Ca concentration, which is used as an important intracellular signal in the enterocyte [ 5 ] as in other cells.
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