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Example sentences for: employing
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The following clustering algorithms were used: seven hierarchical clustering methods (single linkage, average linkage, complete linkage, Ward's minimum variance, density linkage, centroid, and flexible-beta), a k-mean method, and an oblique principle component method employing either (1) Pearson correlation or (2) Spearman correlation based on rank.
Phosphorimage analysis of the radioactivity incorporated in a trans reaction employing 1.2 × 10 9core particles (quantitated as genome-equivalents of viral DNA by slot-blot hybridization) indicated that about 4.8 × 10 10nucleotides were incorporated into DNA in the reaction.
Early studies with high density arrays suggested the use of 25 mer in situ synthesized oligodeoxyribonucleotides [ 1 ] . A more recent study using covalently attached oligodeoxyribonucleotides found that 30- and 35 mer oligodeoxyribonucleotides generated signals two- to five-fold higher than 25 mers, and signals obtained form 60 mers were only two-fold higher than those obtained from 30 mers but ten-fold higher than those obtained from 25 mers [ 24 ] . In fact, in situ synthesized 60 mer oligodeoxyribonucleotides, hybridized in 30-32% formamide, have been shown to represent a compromise between maximal sensitivity and specificity compared to other lengths and hybridization conditions [ 12 ] . Lastly, a study employing
This is demonstrated in Figure 10, employing the same data treated in Figure 9. The advantage of this process is threefold: (1) it treats every data point, not just the time of the peaks; (2) it does not require excessive data conditioning before application of the principal piece of analysis; (3) it obviates the need for judgment calls by the analyst (such as when has the steady state been reached).
LM-PCR has been used in several studies, including a systematic genome-wide location analysis of yeast transcriptional activators [ 10 ] , and an unbiased search for E2F binding sites in a subset of human promoters [ 11 ] . Studies employing the R-PCR approach include genome-wide analyses of silencing enzymes [ 12 ] and histone modifications [ 6 7 ] in yeast, and a mapping of GATA-1 binding sites within the human β-globin locus [ 13 ] .
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