Words similar to elmer
Example sentences for: elmer
How can you use “elmer” in a sentence? Here are some example sentences to help you improve your vocabulary:
The PCR reaction mixture consisted of 1 × buffer A (Perkin-Elmer), 3 mM MgCl 2 , 200 μM dNTPs, 90 nM of each adenovirus primer, 100 nM of each 18S rDNA primer, 200 nM of each probe, 0.6 U AmpliTaq Gold polymerase (Perkin-Elmer) and 5 μl of total DNA sample.
Since etiolation results in rapid elongation of seedlings it was expected that there would be a concomitant increase in subunit A messenger RNA as seen with proteolipid subunit mRNA in A. thaliana [ 36 ] and subunit A message levels in rapidly expanding cotton ( Gossypium hirsutum ) fibers [ 73 ] . Equivalent loading of whole RNA was evaluated via spectrophotometry measuring absorption at 260 nm using a Perkin-Elmer Lambda-3 spectrophotometer and by visual confirmation using non denaturing ethidium bromide stained agarose gel electrophoresis (results not shown).
In general, PCR reactions were performed in a volume of 50 μl with 50 picomoles of each primer, dNTP's (Amersham Pharmacia Biotech, Inc., Piscataway, NJ) at a final concentration of 0.5 mM, 1 μl of template, 1X PCR buffer (10X PCR buffer contained 700 mM Tris pH 8.8, 20 mM MgCl 2 , 1% Triton X-100, and 0.1% Tween 20) and Taq polymerase (Perkin-Elmer Inc., Wellesley MA) diluted to the manufacturers recommendations.
The pol coding region from the recombinant viruses was confirmed to be identical to their respective parental virus pol sequences by terminator cycle sequencing using an automated model 377 DNA sequencer (Perkin Elmer, Applied Biosystems).
Elmers, the nickname for homicidal maniacs similar to the well-publicized one named Elmer .
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