Words similar to electroporated
Example sentences for: electroporated
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L929 cells were electroporated with the GFP-Hyal-1 or GFP-Hyal-2 construct, cultured for 48 hr and then exposed to actinomycin D, daunorubicin or camptothecin for 24 hr.
Untreated HPB-ALL, mock electroporated control HPB-ALL, or C3 treated HPB-ALL do not extend pseudopodia on the non-specific cell attachment substrate poly-L-lysine (figure 3, panels 13-15), demonstrating the requirement for integrin dependent signals in both the normal spreading morphology of HBP-ALL, and the C3 induced morphological changes in this cell type.
100 μg of pHDMCCre8 was introduced into 5 × 10 7ES cells by electroporation using 400 v/500μf/resistance setting 8. 1/100,000 of the total electroporated cell population was plated per 10 cm 2tissue culture dish in ES cell medium supplemented with LIF and grown until individual colonies could be collected.
To generate a library of recombinant plasmids, 200 ng of the ligated sample were electroporated into Electromax DH5α cells.
Although ribosylation of other Rho family proteins such as Rac1 can occur with C3 [ 33 ] , this seemed to be very weak [ 13 ] . In our work, C3 electroporated into the T cell line HPB-ALL caused complete ADP-ribosylation of Rho proteins (~25 kDa).