Words similar to egta

Example sentences for: egta

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Reaction mixtures were then transferred immediately to a 30°C water bath, and proteolysis was inhibited 5 min later by addition of an equal volume of 2× SDS sample buffer containing 2 mM EGTA.
The beads with the bound proteins were washed and then resuspended in 30 μl of kinase buffer [50 mM HEPES, pH 7.4, 2.5 mM EGTA, 10 mM β-glycerophosphate, 0.1 mM vanadate, 1 mM NaF, 1 mM DTT, 10 mM MgCl 2 , 20 μM ATP, 50 μCi γ- 32P-ATP (7000 Ci/mmol, ICN), and 1 μg histone H1].
In order to evaluate whether Btk:ER-induced calcium mobilization was due to calcium release from internal stores or calcium influx across cell membrane calcium release activated channels (CRAC), cells were pre-treated with EGTA to chelate extracellular calcium (Fig 2D; note change in scale within Fig.
5 (100 mM MES, pH 6.5 adjusted with KOH, 1 mM EGTA, 5 mM Mg acetate) and stored at -80°C.
Total RNA from CHO.asbt and BN cells was prepared and analyzed by RNA blotting as previously described [ 17 ] . Cell lysates were prepared by lysing cells in 50 mM Tris-HCl (pH 7.4), 1% NP-40%, 0.25% sodium deoxycholate; 150 mM NaCl, 1 mM EGTA, 1 mM Na 3 VO 4 , 1 mM sodium fluoride, containing a protease inhibitor cocktail (Sigma-Aldrich Canada Ltd.).