Words similar to egta
Example sentences for: egta
How can you use “egta” in a sentence? Here are some example sentences to help you improve your vocabulary:
However, we found that the PIP 2 -stimulated proteolysis of Boi1-PH is strongly enhanced by Ca ++and is inhibited by EGTA (data not shown), suggesting that this protease may require Ca ++for activity.
Control and treated cells were rinsed twice with PBS and then incubated with 1 ml of lysis buffer (10 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1.5 mM MgCl 2 , 50 mM NaF, 5 mM sodium pyrophosphate, 0.2 mM sodium orthovanadate, 2 μg/ml aprotinin, 2 μg/ml leupeptin, 10% glycerol, 1% Triton X-100, 0.5% NP-40, 1 mM phenylmethylsulfonyl fluoride) for 1 hour with shaking at 4°C.
In order to evaluate whether Btk:ER-induced calcium mobilization was due to calcium release from internal stores or calcium influx across cell membrane calcium release activated channels (CRAC), cells were pre-treated with EGTA to chelate extracellular calcium (Fig 2D; note change in scale within Fig.
5 mM, EGTA 0.5 mM, NaF 0.5 mM).
Western blots were performed as described previously [ 13 ] . For all other antibodies, rat intercostal muscle was excised and homogenized in 20 mM HEPES, pH 7.4, with 20 mM Nad, 5 mM EDTA/EGTA, 0.5% Triton X-100, ImM DTT and protease inhibitors on ice.
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