Example sentences for: egta

How can you use “egta” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • All drugs were prepared in calcium-free Locke's solution containing no EGTA.

  • Briefly, retinae were isolated with homogenization buffer (HB, 0.32 mM sucrose, 2 mM Hepes, pH 7.4, 1 mM EGTA, 2 μg/ml leupeptin and 2 μg/mg Aprotinin) and homogenized.

  • To determine if IDO expression was related to the removal of cells from their normal growth substrate or the process of reattachment to new substrate, we trypsinized P19 cells and reseeded them as aggregates in suspension in the presence of various concentrations of EGTA.

  • The SPM proteins were first incubated with 50 μl of protein G-agarose in buffer-1 (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1% Nonidet P40, 0.5% sodium deoxycholate, 2 μg/ml aprotinin, 5 mM EGTA, 4 mM Pefabloc SC, 1 μM pepstatin and 10 μg/ml leupeptin) at 4°C for 3 hours on a rocking platform to reduce background caused by non-specific absorption of irrelevant cellular proteins.

  • For mock micrococcal nuclease treatment, the CaCl 2 micrococcal nuclease and EGTA were mixed prior to addition to the permeabilized cores.


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