Example sentences for: egta

How can you use “egta” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The beads with the bound proteins were washed and then resuspended in 30 μl of kinase buffer [50 mM HEPES, pH 7.4, 2.5 mM EGTA, 10 mM β-glycerophosphate, 0.1 mM vanadate, 1 mM NaF, 1 mM DTT, 10 mM MgCl 2 , 20 μM ATP, 50 μCi γ- 32P-ATP (7000 Ci/mmol, ICN), and 1 μg histone H1].

  • The diagnosis of each patient is shown in Table 1. Tissue samples were homogenized in a Dounce homogenizer on ice in 1 ml of lysis buffer (25 mM MES, 1 mM MgSO 4 , 2 mM EGTA, 0.1 mM GTP, 0.1% Triton X-100 with a protease inhibitor cocktail [Complete Protease Inhibitor Tablets; Boehringer Mannheim]).

  • The medium was removed and the cells were rinsed with 10 ml of cold phosphate-buffered saline (PBS) containing 5 mM EGTA, pH 7.4.

  • Nucleic acids within permeabilized cores were digested with micrococcal nuclease in the presence of CaCl 2 Micrococcal nuclease was inactivated by addition of EGTA, 1.5 μg RNA was added, and the reaction was performed under conditions described for the endogenous polymerase reaction.

  • Yeast cytosol (from 541,000 × g spins) containing wild-type and mutant versions of Boi1-PH were prepared and stored as described for the proteolysis assay, except that the protease inhibitors leupeptin, benzamidine, and EGTA were included.


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