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One approach was to use overlapping BAC genomic sequences to select polymorphisms that varied by more than two repeats [ 15 ] . The other approach was to browse the genome for STRs using the December 12, 2000 version of the genomic sequence at University of California - Santa Cruz http://genome.ucsc.edu/ [ 36 ] .
CoreGenes is written in JAVA-based programming incorporating the 'setdb' and 'BLASTP' programs from the WU-BLAST package of Washington University, http://BLAST.wustl.edu.
1 http://genome.cse.ucsc.edu, no major deletions have been identified except for one report of an exon 60-62 genomic deletion [ 7 ] . Some correlations between FBN1 mutation genotypes and phenotypes are emerging.
Primers were designed with the Primer3 software http://www-genome.wi.mit.edu/cgi-bin/primer/primer3_www.cgiand were synthesized by Sigma-Genosys.
Maximum likelihood pairwise distances are calculated using BLOSUM matrices [ 25 ] by a modified version of TREE-PUZZLE [ 34 ] . Neighbor joining trees are calculated by a modified version of NEIGHBOR from the PHYLIP package [ 35 ] http://evolution.genetics.washington.edu/phylip.html.