Example sentences for: ecotropic

How can you use “ecotropic” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The Phoenix E ecotropic packaging cell line was obtained from Dr Gary Nolan (Stanford University, USA).

  • Murine fibroblast NIH 3T3 cells, amphotropic PA317 packaging cells, and Phoenix E ecotropic packaging cells were cultured in Dulbecco's modified Eagle's medium (DMEM)-high glucose (GIBCO-BRL, Grand island, NY, USA) supplemented with 10% heat-inactivated fetal bovine serum (GIBCO-BRL, Grand island, NY, USA), 100 U/ml penicillin, 100 μg/ml streptomycin, and 200 mM L-glutamine.

  • Following transinfection with ecotropic viral supernatant, 100% of the PA317 cells were transduced with the pRET2.EGFP vector, as determined by fluorescence microscopy.

  • The vector alone, or the antisense BRCA1 vector, was transfected using the calcium-phosphate precipitation method into the ecotropic packaging cell line GPE86 [ 31].

  • The pRET2.EGFP or pRET2.COX2 plasmids were transfected into ecotropic Phoenix E packaging cells by the calcium phosphate precipitation method, using the ProFection kit (Promega, Madison, WI, USA).


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