Example sentences for: e-tp
How can you use “e-tp” in a sentence? Here are some example sentences to help you improve your vocabulary:
The E-TP binary complexes formed were resolved at 4°C on 6% native polyacrylamide gel using Tris-Borate buffer (85 mM Tris, 85 mM Boric acid, pH 8.0).
The preformed E-TP complex was then incubated in the presence of next correct dNTP followed by addition of 300-fold molar excess of unlabeled TP as the DNA trap.
The amounts of the TP in the binary complex (E-TP) and in free form with respect to the varying concentrations of the enzyme protein were determined by PhosphorImager (Molecular Dynamics, Pharmacia) analysis of the gel.
We found that E-TP binary complex was completely competed out by the DNA trap (data not shown) while a significant amount of the E-TP binary complex converted to E-TP-dNTP ternary complex was resistant to competition with DNA trap (Fig.
The chosen concentration of enzyme was such that resulted in almost complete shift during E-TP complex formation.